产品和服务 / 产品类型 / 细胞系&裂解液 / KO细胞系

Recombinant Human FGF-4 Protein (RP01712)

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Recombinant Human FGF-4 Protein was determined by SDS-PAGE under reducing conditions with Coomassie Blue.

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货号: RP01712
价 格：  780.00
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详细信息

靶点
FGF-4
细胞系
19.76 kDa
突变描述
Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.

Mammalian cells such as human, rat and mouse cells are normally diploid with two alleles.
Homozygote: both alleles were knocked out, mRNA has no signal, no expression of proteins.
Heterozygote: only one allele was knocked out, the mRNA transcript levels was decreased compared to wild type, and the protein expression levels was also lower than that of the wild type.
敲除验证
20 kDa
产品组成
Gly25-Leu206
物种
E. coli
保存条件
Store at -20℃
运输条件
Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.

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折叠内容
中国科学院动物研究所Nov 282025
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Experiment Type
Cell-based assay
Sample
hPSC
Description
The differentiation medium containing Wnt-3a (500 ng/mL) and FGF-4 (500 ng/mL) was used to induce the differentiation of definitive endoderm (DE) cells into mid-hindgut cells. After 4 days of induction, the expression of the mid-hindgut marker CDX2 was assessed by immunofluorescence.
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背景信息

FGF (fibroblast growth factor) signalling is known to be required for many aspects of mesoderm formation and patterning during Xenopus development and has been implicated in regulating genes required for the specification of both blood and skeletal muscle lineages. Fibroblast growth factor 4 (FGF4) signaling induces differentiation from embryonic stem cells (ESCs) via the phosphorylation of downstream molecules such as mitogen-activated protein kinase/extracellular signal-related kinase (MEK) and extracellular signal-related kinase 1/2 (ERK1/2). Fibroblast Growth Factor 4 (FGF-4) could not only increase the proliferation of bone marrow mesenchymal stem cells (BMSCs), but also induce BMSCs into hepatocyte-like cells in vitro. FGF4 transduced BMSCs contributed to liver regeneration might by the transplanted microenvironment. The FGF4-bFGF BMSCs thus can enhance the survival of the transplanted cells, diminish myocardial fibrosis, promote myocardial angiogenesis, and improve cardiac functions.

基因ID
2249
Swiss Prot
P08620-1
别名
HST; KFGF; FGF-4; HST-1; HSTF1; K-FGF; HBGF-4; HSTF-1;FGF4