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活性验证

Recombinant Human Wnt-3a Surrogate Protein (RP01618SLQ)

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Recombinant Human Wnt-3a Surrogate Protein was determined by SDS-PAGE under reducing conditions with Coomassie Blue.

Recombinant Human Wnt-3a Surrogate Protein is greater than 95% as determined by SEC-HPLC.

Measured by its ability to induce Topflash reporter activity in HEK293T human embryonic kidney cells. The ED50 for this effect is 7.38-29.54 ng/mL ,corresponding to a specific activity of 3.39×104~1.35×105 units/mg.

Human stomach organoids organoids were cultured with EGF(Cat. RP03287), FGF10(Cat. RP01140), NOG(Cat. RP01237), RSPO1(Cat. RP00071), WNT-3a(Cat. RP01618SLQ).

Mouse large intestinal organoids were cultured with EGF(Cat. RP03287), NOG(Cat. RP01237), RSPO1(Cat. RP00071), WNT-3a(Cat. RP01618SLQ)

Human liver organoids were cultured with EGF(Cat. RP03287), HGF(Cat. RP01602), FGF2(Cat. RP01042), FGF10(Cat. RP01140), NOG(Cat. RP01237), RSPO1(Cat. RP00071), WNT-3a(Cat. RP01618SLQ).

Human kidney organoids were cultured with EGF(Cat. RP03287), FGF2(Cat. RP01042), FGF7(Cat. RP01717), FGF9(Cat. RP01710), FGF10(Cat. RP01140), IGF-(Cat. RP00996), NOG(Cat. RP01237), RSPO1(Cat. RP00071), WNT-3a(Cat. RP01618SLQ). And further, DKK-1(RP01343) was used to induce the establishment of cell polarity.

All(7)|
货号: RP01618SLQ
促销价：   ￥950
货    期：现货产品
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详细信息

种属
Human
表达宿主
HEK293 Cells
Calculated MW
67.52 kDa
Observed MW
66-75 kDa
标签
C-hFc
纯度
≥ 95 % as determined by SDS-PAGE;≥ 95% as determined by HPLC.
内毒素
< 0.01 EU/μg of the protein by LAL method
制剂
Lyophilized from sterile 0.22 μm filtered solution in PBS buffer with Trehalose, Glycerol, and Tween80, pH 7.4.
描述
Recombinant Human Wnt-3a Surrogate Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence of Human Wnt-3a Surrogate fused with hFC at the C-terminus.
储存
Store at -70°C. This product is stable at ≤ -70°C for up to 1 year from the date of receipt. For optimal storage, aliquot into smaller quantities after centrifugation and store at recommended temperature. Avoid repeated freeze-thaw cycles.未开盖的干粉蛋白在 -20°C至-80°C可保存12个月；
复溶之后，蛋白溶液在-20°C及以下可保存3个月，在2-8℃可保存1周。
生物活性
1、Measured by its ability to induce Topflash reporter activity in HEK293T human embryonic kidney cells. The ED50 for this effect is 7.3829.54 ng/mL ,corresponding to a specific activity of 3.39×104~1.35×105 units/mg.

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客户数据及评论 (1)

折叠内容
厦门百脑慧医学研究有限公司Jul 282025
评分
Experiment Type
Cell-based Assay
Sample
prostate cancer cells
Description
The picture show culture of human prostate cancer cells using Wnt-3a (RP01618SLQ), Noggin (RP01237), FGF-10 (RP01140), bFGF (RP01042), RSPO1 (RP00071), and EGF (RP01030).

背景信息

Wnt3a Surrogate protein is a Wnt recombination protein that plays an important role in embryonic development, adult tissue homeostasis and injury repair. Wnt3a Surrogate protein, consisting of the domain and Fc portion of Wnt3a, helps organoids expand and maintain a source of Wnt. Organoids are a rapidly evolving model system for tissue development, and Wnt3a is considered as the essential protein for organoid growth. Wnt3a or the small molecule glycogen synthase kinase 3 (GSK3) inhibitor CHIR are commonly used as Wnt sources in experiments. However, the application of Wnt3a is hampered by Wnt lipidation, and cross-reactivity with Wnt frizzled (Fzd). Therefore, Wnt3a Surrogate protein was used instead of Wnt3a, as a water-soluble and Fzd-specific alternative Wnt agonist.

别名
WNT3A;Protein Wnt-3a;Wnt3a Surrogate