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Active Recombinant Mouse CSF-1/M-CSF Protein (RP01216)

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Active Recombinant Mouse CSF-1/M-CSF Protein was determined by SDS-PAGE with Coomassie Blue, showing a band at 40-55 kDa.

Recombinant Mouse M-CSF promotes the proliferation of M-NFS-60 mouse myelogenous leukemia lymphoblast cells. The ED50 for this effect is typically 0.1-0.4 ng/mL, corresponding to a specific activity of 2.5×106-1.0×107units/mg.

Recombinant Mouse CSF-1/M-CSF stimulates cell proliferation of the mouse bone marrow cells. The ED50 for this effect is 7.5-30.1 ng/mL, corresponding to a specific activity of 3.32×104~1.33×105 units/mg.

Recombinant mouse M-CSF (20 ng/mL) induces mouse primary Bone Marrow cells to differentiate into Macrophages(BMDM). After 7 days, the induction was successful according to cell morphology.(Customer feedback data)

Recombinant mouse M-CSF (20 ng/mL) to induce the mice bone marrow cells for 7 days and then perform flow cytometry to detect the signals of M0 macrophage markers CD11b and F4/80, both are increased. (Customer feedback data)

All(5)|
货号: RP01216
促销价：   ￥1100
货    期：现货产品
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文献引用 (1)

折叠内容

Publishing research using RP01216? Please let us know so that we can cite the reference in this datasheet.

详细信息

种属
Mouse
表达宿主
HEK293 cells
描述
Active Recombinant Mouse CSF-1/M-CSF Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Met1-Glu262) of mouse M-CSF/CSF-1 (Accession #NP_031804.3.) fused with a 6×His tag at the C-terminus.
标签
C-His
纯度
> 95% by SDS-PAGE.
内毒素
< 0.1 EU/μg of the protein by LAL method.
生物活性
1.Measured in a cell proliferation assay using M-NFS-60 mouse myelogenous leukemia lymphoblast cells. The ED50 for this effect is typically 0.1-0.4 ng/mL, corresponding to a specific activity of 2.5×106-1.0×107units/mg.
2.Measured in a cell proliferation assay using mouse bone marrow cells. The ED50 for this effect is 7.5-30.1 ng/mL, corresponding to a specific activity of 3.32×104~1.33×105 units/mg.
3.Measured in a cell proliferation assay using M-NFS-60 mouse myelogenous leukemia lymphoblast cells. The ED50 for this effect is 2-8 ng/mL, corresponding to a specific activity of 12.5×104~5.0×105 units/mg.
制剂
Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.Contact us for customized product form or formulation.
储存
Store the lyophilized protein at -20°C to -80 °C for long term.
After reconstitution, the protein solution is stable at -20 °C for 3 months, at 2-8 °C for up to 1 week.未开盖的干粉蛋白在 -20°C至-80°C可保存12个月；
复溶之后，蛋白溶液在-20°C及以下可保存3个月，在2-8℃可保存1周。
复溶
Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid votex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.收到重组蛋白产品之后请检查蛋白冻干粉末是否贴于瓶底，如果粉末浮起，开盖之前请先低温离心。将蛋白用说明书中指定的缓冲液复溶至0.1-0.5 mg/mL（请注意蛋白复溶浓度不能低于0.1 mg/mL），室温平衡5-10 min保证充分溶解，复溶过程中请不要剧烈涡旋及吹打蛋白溶液。如需长期储存，建议复溶时添加载体蛋白或者稳定剂（如0.1% BSA, 5% HSA, 10% FBS 或者 5% 海藻糖），同时将复溶后的蛋白溶液按照需求进行分装，储存于-20°C至-80°C，随取随用，避免反复冻融。

蛋白复溶计算器

请在蛋白复溶计算器中输入蛋白总质量和所需终浓度，快速计算您需要添加溶液的体积吧！
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背景信息

Macrophage colony-stimulating factor 1, also known as CSF-1, M-CSF, is a single-pass membrane protein which is disulfide-linked as a homodimer or heterodimer. Granulocyte / macrophage colony-stimulating factors are cytokines that act in hematopoiesis by controlling the production, differentiation, and function of 2 related white cell populations of the blood, the granulocytes and the monocytes-macrophages. M-CSF/CSF-1 is known to facilitate monocyte survival, monocyte-to-macrophage conversion, and macrophage proliferation. M-CSF/CSF-1 is a secreted cytokine which influences hemopoietic stem cells to differentiate into macrophages or other related cell types. It binds to the Colony stimulating factor 1 receptor. M-CSF/CSF-1 may also be involved in development of the placenta. The active form of M-CSF/CSF-1 is found extracellularly as a disulfide-linked homodimer, and is thought to be produced by proteolytic cleavage of membrane-bound precursors. M-CSF/CSF-1 induces cells of the monocyte/macrophage lineage. It also plays a role in immunological defenses, bone metabolism, lipoproteins clearance, fertility and pregnancy. Upregulation of M-CSF/CSF-1 in the infarcted myocardium may have an active role in healing not only through its effects on cells of monocyte/macrophage lineage, but also by regulating endothelial cell chemokine expression.

基因ID
12977
Swiss Prot
P07141-1
别名
MCSF;M-CSF;CSF-1;Lanimostim;CSF1
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