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Recombinant Human CD44 Protein (RP00973)

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Recombinant Human CD44 Protein was determined by SDS-PAGE with Coomassie Blue, showing a band at 70-85 kDa.

Immobilized Hyaluronic Acid at 100 μg/mL (100 μL/well) can bind Recombinant Human CD44 with a linear range of 20-330 ng/mL.

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货号: RP00973
促销价：   ￥620
货    期：现货产品
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文献引用 (2)

折叠内容

Publishing research using RP00973? Please let us know so that we can cite the reference in this datasheet.

详细信息

种属
Human
表达宿主
HEK293 cells
描述
Recombinant Human CD44 Protein is produced by HEK293 expression system. The target protein is expressed with sequence (Gln21-Pro220) of human CD44 (Accession #NP_000601.3) fused with an Fc, 6×His tag at the C-terminus.
标签
C-hFc&His
纯度
> 97% by SDS-PAGE.
内毒素
< 1.0 EU/μg of the protein by LAL method.
生物活性
Measured by its binding ability in a functional ELISA. Immobilized Hyaluronic Acid at 100 μg/mL (100 μL/well) can bind Human CD44 with a linear range of 20-330 ng/mL.
制剂
Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.Contact us for customized product form or formulation.
储存
Store the lyophilized protein at -20°C to -80 °C for long term.
After reconstitution, the protein solution is stable at -20 °C for 3 months, at 2-8 °C for up to 1 week.未开盖的干粉蛋白在 -20°C至-80°C可保存12个月；
复溶之后，蛋白溶液在-20°C及以下可保存3个月，在2-8℃可保存1周。
复溶
Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid votex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.收到重组蛋白产品之后请检查蛋白冻干粉末是否贴于瓶底，如果粉末浮起，开盖之前请先低温离心。将蛋白用说明书中指定的缓冲液复溶至0.1-0.5 mg/mL（请注意蛋白复溶浓度不能低于0.1 mg/mL），室温平衡5-10 min保证充分溶解，复溶过程中请不要剧烈涡旋及吹打蛋白溶液。如需长期储存，建议复溶时添加载体蛋白或者稳定剂（如0.1% BSA, 5% HSA, 10% FBS 或者 5% 海藻糖），同时将复溶后的蛋白溶液按照需求进行分装，储存于-20°C至-80°C，随取随用，避免反复冻融。

蛋白复溶计算器

请在蛋白复溶计算器中输入蛋白总质量和所需终浓度，快速计算您需要添加溶液的体积吧！
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背景信息

This protein is a cell-surface glycoprotein involved in cell-cell interactions, cell adhesion and migration. It is a receptor for hyaluronic acid (HA) and can also interact with other ligands, such as osteopontin, collagens, and matrix metalloproteinases (MMPs). This protein participates in a wide variety of cellular functions including lymphocyte activation, recirculation and homing, hematopoiesis, and tumor metastasis. Transcripts for this gene undergo complex alternative splicing that results in many functionally distinct isoforms, however, the full length nature of some of these variants has not been determined. Alternative splicing is the basis for the structural and functional diversity of this protein, and may be related to tumor metastasis.

基因ID
960
Swiss Prot
P16070-1
别名
CD44; CDW44; CSPG8; ECMR-III; HCELL; HUTCH-I; IN; LHR; MC56; MDU2; MDU3; MIC4; Pgp1; CD44 antigen;CDW44;CSPG8;ECMR-III;HCELL;HUTCH-I;IN;LHR;MC56;MDU2;MDU3;MIC4;Pgp1;CD44
研究领域
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