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Recombinant Mouse Fc-gamma RIII/CD16 Protein (RP00761)

Recombinant Mouse Fc-gamma RIII/CD16 Protein was determined by SDS-PAGE under reducing conditions with Coomassie Blue.

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货号: RP00761
促销价:   ¥760
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折叠内容

Publishing research using RP00761? Please let us know so that we can cite the reference in this datasheet.

详细信息

种属
Mouse
表达宿主
HEK293 cells
描述
Recombinant Mouse Fc-gamma RIII/CD16 Protein is produced by Human cells expression system. The target protein is expressed with sequence (Ala31-Thr215) of mouse Fc γ RIIIA/FCGR3/CD16 (Accession #P08508) fused with a 6×His tag at the C-terminus.
标签
C-His
纯度
> 95% by SDS-PAGE.
内毒素
< 1 EU/μg of the protein by LAL method.
制剂
Lyophilized from a 0.2 μm filtered solution of PBS, pH7.4.Contact us for customized product form or formulation.
储存
Store the lyophilized protein at -20°C to -80 °C for long term.
After reconstitution, the protein solution is stable at -20 °C for 3 months, at 2-8 °C for up to 1 week.未开盖的干粉蛋白在 -20°C至-80°C可保存12个月;
复溶之后,蛋白溶液在-20°C及以下可保存3个月,在2-8℃可保存1周。
复溶
Centrifuge the tube before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid votex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.收到重组蛋白产品之后请检查蛋白冻干粉末是否贴于瓶底,如果粉末浮起,开盖之前请先低温离心。将蛋白用说明书中指定的缓冲液复溶至0.1-0.5 mg/mL(请注意蛋白复溶浓度不能低于0.1 mg/mL),室温平衡5-10 min保证充分溶解,复溶过程中请不要剧烈涡旋及吹打蛋白溶液。如需长期储存,建议复溶时添加载体蛋白或者稳定剂(如0.1% BSA, 5% HSA, 10% FBS 或者 5% 海藻糖),同时将复溶后的蛋白溶液按照需求进行分装,储存于-20°C至-80°C,随取随用,避免反复冻融。

蛋白复溶计算器

请在蛋白复溶计算器中输入蛋白总质量和所需终浓度,快速计算您需要添加溶液的体积吧!
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背景信息

Low affinity immunoglobulin gamma Fc region receptor III (Fc gamma RIII/CD16) is a member of the Igsuperfamily. Based on close relationships in their extracellular domains, the Fc gamma Rs have been dividedinto three classes composing of Fc gamma RI (CD64), Fc gamma RII (CD32), and Fc gamma RIII (CD16). Eachgroup may be encoded by multiple genes and exist in different isoforms depending on species and cell type.Mouse CD16 is a type I transmembrane protein having two extracellular Ig-like domains consisting ofimmunoglobulin domain, repeat, signa and transmembrane, transmembrane helix. It is expressed on a varietyof myeloid and lymphoid cells and associates with Fc R gamma to deliver an activating signal upon ligandbinding. Fcgr3 is IgG binding and activation or inhibition of immune responses such as antibody-dependentcellular cytotoxicity, phagocytosis, cell surface receptor signaling pathway and positive regulation of typeI/IIa/III hypersensitivity.

Swiss Prot
别名
Low affinity immunoglobulin gamma Fc region receptor III; Fcgr3; Fc gamma Receptor III; CD-antigen 16; CD16; FcRIII; IgG Fc receptor III
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