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活性验证

Recombinant Human R-spondin-1/RSPO1 Protein (RP00071)

评论 (7) 说明书 收藏

Recombinant Human R-spondin-1/RSPO1 Protein was determined by SDS-PAGE under reducing conditions with Coomassie Blue.

The purity of Recombinant Human R-spondin-1/RSPO1 Protein is greater than 90% as determined by SEC-HPLC.

Recombinant Human R-Spondin1 enhances Cyclin D1 expression in HCT116 human colon adenocarcinoma cells. 0.1-10ng/mL of Recombinant Human RSPO1 can effectively enhance Cyclin D1 expression.

Recombinant Human R-spondin-1/RSPO1 Protein induce Topflash reporter activity in HEK293T human embryonic kidney cells. The ED50 for this effect is 6.61~26.44 ng/mL in the presence of 5 ng/mL Wnt Surrogate Protein, corresponding to a specific activity of 3.78×104~1.51×105 units/mg.

Human stomach organoids were cultured with EGF(Cat. RP03287), FGF10(Cat. RP01140), NOG(Cat. RP01237), RSPO1(Cat. RP00071), WNT-3a(Cat. RP01618SLQ).

Mouse large intestinal organoids were cultured with EGF(Cat. RP03287), NOG(Cat. RP01237), RSPO1(Cat. RP00071), WNT-3a(Cat. RP01618SLQ)

Mouse small intestinal organoids were cultured with EGF(Cat. RP03287), NOG(Cat. RP01237), RSPO1(Cat. RP00071).

Human liver organoids were cultured with EGF(Cat. RP03287), HGF(Cat. RP01602), FGF2(Cat. RP01042), FGF10(Cat. RP01140), NOG(Cat. RP01237), RSPO1(Cat. RP00071), WNT-3a(Cat. RP01618SLQ).

Human kidney organoids were cultured with EGF(Cat. RP03287), FGF2(Cat. RP01042), FGF7(Cat. RP01717), FGF9(Cat. RP01710), FGF10(Cat. RP01140), IGF-(Cat. RP00996), NOG(Cat. RP01237), RSPO1(Cat. RP00071), WNT-3a(Cat. RP01618SLQ). And further, DKK-1(RP01343) was used to induce the establishment of cell polarity.

All(9)|
货号: RP00071

促销价：   ￥780
货    期：现货产品
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详细信息

种属
Human
表达宿主
HEK293 cells
Calculated MW
26.44 kDa
Observed MW
39 kDa
标签
C-His
纯度
≥ 95 % as determined by SDS-PAGE;≥ 90 % as determined by HPLC.
内毒素
< 0.1 EU/μg of the protein by LAL method.
制剂
Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.Contact us for customized product form or formulation.
描述
Recombinant Human R-spondin-1/RSPO1 Protein is produced by HEK293 expression system. The target protein is expressed with sequence (Arg31-Ala263) of human R-Spondin1 (Accession #NP_001033722.1) fused with a 6×His tag at the C-terminus.
储存
Store at -20℃.Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt.
After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week.未开盖的干粉蛋白在 -20°C至-80°C可保存12个月；
复溶之后，蛋白溶液在-20°C及以下可保存3个月，在2-8℃可保存1周。
生物活性
1.Measured by its ability to enhance Cyclin D1 expression in HCT116 human colon adenocarcinoma cells. 0.1-10ng/mL of Recombinant Human RSPO1 can effectively enhance Cyclin D1 expression.
2.The intestinal crypts of mice were cultured in organoid culture medium containing factor combinations (100 ng/mL Noggin, Cat. RP01237 + 500 ng/mL R-spindin-1, Cat. RP00071) derived from ABclonal for144 hours, intestinal organoids were formed. (Customer Feedback Data)
3.Recombinant Human R-Spondin 1 protein stimulated Wnt signal pathway with Wnt-3a protein in HEK293T cells. After 6 hours, the stimulation when adding 300 ng/mL of R-Spondin-1 reached highest effect. Compared with only Wnt-3a stimulation, the Wnt signaling pathway was enhanced 3.1-fold after adding 300 ng/mL R-Spondin 1.
复溶
Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.收到重组蛋白产品之后请检查蛋白冻干粉末是否贴于瓶底，如果粉末浮起，开盖之前请先低温离心。将蛋白用说明书中指定的缓冲液复溶至0.1-0.5 mg/mL（请注意蛋白复溶浓度不能低于0.1 mg/mL），室温平衡5-10 min保证充分溶解，复溶过程中请不要剧烈涡旋及吹打蛋白溶液。如需长期储存，建议复溶时添加载体蛋白或者稳定剂（如0.1% BSA, 5% HSA, 10% FBS 或者 5% 海藻糖），同时将复溶后的蛋白溶液按照需求进行分装，储存于-20°C至-80°C，随取随用，避免反复冻融。

蛋白复溶计算器

请在蛋白复溶计算器中输入蛋白总质量和所需终浓度，快速计算您需要添加溶液的体积吧！
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客户数据及评论 (7)

折叠内容
厦门客户Aug 212025
评分
Experiment Type
Cell-based assay
Sample
mouse small intestinal organoids, mouse bile duct organoids, mouse colonic organoids, and human colorectal cancer organoids
Description
RSPO1 is used in the cultivation of mouse small intestinal organoids, mouse bile duct organoids, mouse colonic organoids, and human colorectal cancer organoids, with the culture effects and status being comparable to the control.
厦门百脑慧医学研究有限公司Jul 282025
评分
Experiment Type
Cell-based Assay
Sample
prostate cancer cells
Description
The picture show culture of human prostate cancer cells using Wnt-3a (RP01618SLQ), Noggin (RP01237), FGF-10 (RP01140), bFGF (RP01042), RSPO1 (RP00071), and EGF (RP01030).
南京客户Jun 112025
评分
Experiment Type
Cell-based assay
Sample
tissue
Description
Animal tissues-derived intestinal stem cells were cultured in organoid culture medium containing factor combinations (WNT-3A, EGF, R-spondin-1, Noggin, and matrix gel), and then small intestinal organoids were formed.
厦门大学Nov 122024
评分
Experiment Type
Cell-based assay
Sample
Intestinal crypts
Description
Mouse intestinal crypts were cultured with organoid culture medium supplemented with recombinant human NOG (100 ng/mL, Cat. RP01237) and R-spondin-1 (500 ng/mL, Cat. RP00071) for 132 h and 144 h. And mouse intestinal organoid were obtained.
厦门大学Nov 122024
评分
Experiment Type
Cell-based assay
Sample
293T
Description
Different concentration (100 ng/mL, 300 ng/mL, 500 ng/mL) of R-spondin-1 (Cat. RP00071) were used to treat 293T cells for 6 h. The expression of related gene was detected by luciferase assay to reflect the potency of R-spondin-1 enhancing Wnt pathway signal. At the concentration of 300 ng/mL, the potency of R-spondin-1 reached the highest, and the wnt pathway signal was enhanced by 3.1 times compared with the wnt3a stimulation alone.
复旦大学Nov 122024
评分
Experiment Type
Cell-based assay
Sample
Cell
Description
The picture shows the culture results of mESo (mouse esophageal organoids). R-Spondin1 (Cat. RP00071), Noggin (Cat. RP01237) and EGF (Cat. RP01502) were used during the organoids culture and the final concentration were 200，100，50 ng/mL respectively.
中国中医科学院医学实验中心Oct 232024
评分
Experiment Type
Cell-based assay
Sample
tumor tissue
Description
Patient tissue-derived kidney tumor cells were cultured in organoid culture medium containing factor combinations (100 ng/mL Noggin, Cat. RP01237 + 500 ng/mL R-spindin-1, Cat. RP00071 + 20 ng/mL FGF-10, Cat. RP01140 + 50 ng/mL EGF, Cat. RP01030), and then kidney tumor organoids were formed.

背景信息

This protein is a secreted activator protein with two cysteine-rich, furin-like domains and one thrombospondin type 1 domain. The encoded protein is a ligand for leucine-rich repeat-containing G-protein coupled receptors (LGR proteins) and positively regulates the Wnt signaling pathway. In mice, the protein induces the rapid onset of crypt cell proliferation and increases intestinal epithelial healing, providing a protective effect against chemotherapy-induced adverse effects.

基因ID
284654
Swiss Prot
Q2MKA7
别名
RSPO1;CRISTIN3;RSPO