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Recombinant Human IL-1RN Protein (RP00003)

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Recombinant Human IL-1RN Protein was determined by SDS-PAGE with Coomassie Blue, showing a band at 21 kDa.

Immobilized Recombinant Human IL1RN at 10μg/mL (100 μL/well) can bind Recombinant Human IL1R2 with a linear range of 0.4-1.8 μg/mL.

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货号: RP00003
促销价：   ￥500
货    期：现货产品
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详细信息

种属
Human
表达宿主
E. coli
描述
Recombinant Human IL-1RN Protein is produced by E. coli expression system. The target protein is expressed with sequence (Arg26-Glu177) of human IL-1RN (Accession #NP_776214.1) fused with an initial Met at the N-terminus and a 6×His tag at the C-terminus.
标签
C-His
纯度
> 97% by SDS-PAGE.
内毒素
< 0.1 EU/μg of the protein by LAL method.
生物活性
Measured by its binding ability in a functional ELISA. Immobilized Recombinant Human IL1RN at 10 μg/mL (100 μL/well) can bind Recombinant Human IL1R2 with a linear range of 0.4-1.8 μg/mL.
制剂
Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.Contact us for customized product form or formulation.
储存
Store the lyophilized protein at -20°C to -80 °C for long term.
After reconstitution, the protein solution is stable at -20 °C for 3 months, at 2-8 °C for up to 1 week.未开盖的干粉蛋白在 -20°C至-80°C可保存12个月；
复溶之后，蛋白溶液在-20°C及以下可保存3个月，在2-8℃可保存1周。
复溶
Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid votex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.收到重组蛋白产品之后请检查蛋白冻干粉末是否贴于瓶底，如果粉末浮起，开盖之前请先低温离心。将蛋白用说明书中指定的缓冲液复溶至0.1-0.5 mg/mL（请注意蛋白复溶浓度不能低于0.1 mg/mL），室温平衡5-10 min保证充分溶解，复溶过程中请不要剧烈涡旋及吹打蛋白溶液。如需长期储存，建议复溶时添加载体蛋白或者稳定剂（如0.1% BSA, 5% HSA, 10% FBS 或者 5% 海藻糖），同时将复溶后的蛋白溶液按照需求进行分装，储存于-20°C至-80°C，随取随用，避免反复冻融。

蛋白复溶计算器

请在蛋白复溶计算器中输入蛋白总质量和所需终浓度，快速计算您需要添加溶液的体积吧！
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背景信息

Interleukin-1 receptor antagonist (IL-1RA) also known as IL1RN is a member of the interleukin 1 cytokine family. This protein inhibits the activities of interleukin 1, alpha (IL1A) and interleukin 1, beta (IL1B), and modulates a variety of interleukin 1 related immune and inflammatory responses. A polymorphism of this protein is reported to be associated with increased risk of osteoporotic fractures and gastric cancer.

基因ID
3557
Swiss Prot
P18510
别名
DIRA; ICIL-1RA; IL-1RN; IL-1ra; IL-1ra3; IL1F3; IL1RA; IRAP; MVCD4;IL1RN;ICIL-1RA;IL-1RN;IL-1ra;IL-1ra3;IL1F3;IL1RA;IRAP;MVCD4
研究领域
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