The Color Mixed Protein Marker 180 plus is a ready to use three-color protein standard with 10 pre-stained proteins covering a wide range of molecular weights from 10 to 180 kDa(9 to
170 kDa in Bis-Tris(MOPS)buffer ana 10 to 170 kDa in Bis-Tris(MES)buffer).Proteins are covalently coupled with ablue chromophore except for two reference bands(one green ana one rea band at 25 kDa ana 75 kDa respectively)when separated on SDS PAGE(Aris Glycine buffer).This marker is designed for monitoring protein separation during SDS-polyacrylamide gel electrophoresis verification of Western transfer efficiency on membranes(PVDF,nylon,or nitrocellulose)and for approximating the size of proteins.
产品特点
Economical savings: 3-5 μL single-well loading
Rich colors: three-color pre-staining (green, red, and blue)
Easy to use: direct sample loading (no need to boil heat)
Stable quality: 2 years shelf life (3 months at 4°C, 2 years at -20°C)
Wide range of bands: many bands, wide range (10-180KD: Tris-Glycine /Bis-Tris (MES) buffer, 9-170KD: Bis-Tris (MOPS) buffer)
Precise: Ultra-pure and precision-calibrated proteins as standard
Clear bands: bright colors and neat bands that are visible throughout.
产品应用
Protein marker
产品组成
Product Name
Catalog No.
Size
ColorMixed Protein Marker 180 (10-180 kDa)
RM19001
250 μL/2×250 μL/10×250 μL
Instruction
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1
注意事项
Ready to use:DO NOT heat,dilute,or add reducing agents before loading;
reference bands: 70kDa(Red),25kDa(Green);
Loading Volume:3-5 μl/well for mini gel;6-10 μl/wellfor large gel;