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This standard curve is only for demonstration purposes. A standard curve should be generated for eachassay.
No significant cross-reactivity or interference was observed.
Placed at 37°C for 3 days, the stability of the standard curve all conformto CV <10%.
* This package insert must be read in its entirety before using this product.
Mouse IL-10 Sanwich ELISA Immunoassay
| Recommended Concentration | Sample | |
|---|---|---|
| ELISA Capture | 0.5-2ug/mL | Rabbit anti-Mouse IL-10 mAb(CAP)(Cat. No.RM17935) |
| ELISA Detection | 0.015-0.06ug/mL | Rabbit anti-Mouse IL-10 mAb(DET)(Cat. No.RM17936) |
| Standard | 31.25-2000pg/mL | Recombinant Mouse IL-10 Protein |
Interleukin 10 (IL-10), also known as cytokine synthesis inhibitory factor (CSIF), is a pleiotropic cytokine that can exert immunosuppressive or immunostimulatory effects in a variety of cell types. The human, rat and mouse IL-10 cDNA sequences all contain 178 amino acid residues and contain an 18 amino acid signal peptide sequence, with the mature IL-10 molecule containing 160 amino acid residues and the mouse and human IL-10 molecules containing 5 and 4 cysteine residues, respectively, with a molecular weight of 35-40 kDa. The mouse and human IL-10 genes are located on chromosome 1 and their genomes consist of Both mouse and human IL-10 genes are located on chromosome 1, with a genome consisting of 5 exons and 4 introns. Human and mouse IL-10 share 81% and 73% homology at the DNA and amino acid levels, respectively. Human IL-10 acts on murine-derived cells, whereas mouse IL-10 does not cross-react with human cells.Translated with www.DeepL.com/Translator (free version)
