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This standard curve is only for demonstration purposes. A standard curve should be generated for each assay.
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Human IL-10 multiplex assay
| Recommended Concentration | Sample | |
|---|---|---|
| ELISA Capture | 10ug/106 | Rabbit anti-Human IL-10 mAb (CAP)(Cat. No. RM17643) |
| ELISA Detection | 0.5-2ug/mL | Rabbit anti-Human IL-10 mAb (DET)(Cat. No. RM17644) |
| Standard | 1.4-1000pg/mL | Recombinant IL-10 Protein (Cat. No. RM94521) |
Major immune regulatory cytokine that acts on many cells of the immune system where it has profound anti-inflammatory functions, limiting excessive tissue disruption caused by inflammation. Mechanistically, IL10 binds to its heterotetrameric receptor comprising IL10RA and IL10RB leading to JAK1 and STAT2-mediated phosphorylation of STAT3.In turn, STAT3 translocates to the nucleus where it drives expression of anti-inflammatory mediators.Targets antigen-presenting cells (APCs) such as macrophages and monocytes and inhibits their release of pro-inflammatory cytokines including granulocyte-macrophage colony-stimulating factor /GM-CSF, granulocyte colony-stimulating factor/G-CSF, IL-1 alpha, IL-1 beta, IL-6, IL-8 and TNF-alpha.Interferes also with antigen presentation by reducing the expression of MHC-class II and co-stimulatory molecules, thereby inhibiting their ability to induce T cell activation.In addition, controls the inflammatory response of macrophages by reprogramming essential metabolic pathways including mTOR signaling (By similarity).
