The ColorMixed Protein Marker 250 is a ready to use two-color protein standard with 11
pre-stained proteins covering a wide range of molecular weights from 10 to 250 kDa (10 to
230 kDa in Bis-Tris (MOPS) buffer and 9.8 to 230 kDa in Bis-Tris (MES) buffer). Proteins are covalently coupled with a blue chromophore except for two reference bands (two red bands at 25 kDa and 70 kDa respectively) when separated on SDS-PAGE (Tris-Glycine buffer). This marker is designed for monitoring protein separation during SDS-polyacrylamide gel
electrophoresis, verification of Western transfer efficiency on membranes, (PVDF, nylon, or nitrocellulose) and for approximating the size of proteins.
产品应用
Marker
注意事项
1. Ready to use: Do NOT heat, dilute, or add reducing agents before loading
2.Reference bands: 70 kDa (red), 25 kDa (red)
3.Loading Volume: 3-5 uL/well for mini gel; 1.5-2.5 μL/ well for Western transfer; 6-10 μL/ well for large gel
4.Type of membranes: PVDF, nylon, or nitrocellulose
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