ABscript II Reverse transcriptase is a recombinant M-MuLV reverse transcriptase with low RNase H activity and enhanced thermal stability.Compared to wild-type M-MuLV,recombinant M-MuLV reverse transcriptase can synthesize first-strand cDNA at higher temperatures.The enzyme is active at up to 48℃ with higher specificity and cDNA yield.The kit provides two reverse transcription primers.The Oligo-dT primer [d(T)<sub>23</sub>VN] forces the primer to anneal to the beginning of the polyA tail.The optimized random primer premix has low specificity,and all RNA,including mRNA,rRNA,and tRNA can be used as a reverse transcription template.This kit can amplify cDNA up to 10 kb.
The low activity of RNase H is beneficial to the synthesis of long fragment cDNA, which can synthesize cDNA up to 10 KB.
cDNA first-strand Synthesis
Components | 50 RXN | 100 RXN |
| ABScript II Enzyme Mix (10X) | 100 µL | 200 µL |
| ABScript II Reaction Mix (2X) | 600 µL | 1.25 mL |
| Oligo d(T)<sub>23</sub>VN （50 μM） | 100 µL | 200 µL |
| Random Primer Mix (60 μM) | 100 µL | 200 µL |
| dNTPs (10 mM each) | 50 µL | 100 µL |
| Nuclease-free H<sub>2</sub>O | 1.25 mL | 1.25 mL |
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