| 试剂管名称 | 24 RXN | 96 RXN |
| 2X Frag/Elute Buffer | 144 μL | 576 μL |
| RT Strand Specificity Reagent | 192 μL | 768 μL |
| First Strand Synthesis Enzyme Mix | 48 μL | 192 μL |
| Second Strand Synthesis Reaction Buffer with dUTP | 192 μL | 768 μL |
| Second Strand Synthesis Enzyme Mix | 96 μL | 384 μL |
| Nuclease-free Water | 1 ml X 2 | 8 mL |
| End-prep Buffer | 240 μL | 960 μL |
| End-prep Enzyme Mix | 72 μL | 288 μL |
| Ligation Buffer | 396 μL | 1584 μL |
| Ligase Mix | 72 μL | 288 μL |
| 2X PCR Mix | 600 μL | 1200 μl X 2 |
| UDG Enzyme | 12 μL | 48 μL |
| Low EDTA TE | 1 mL X 3 | 10 mL |
使用100ng 和 1 μg 的Total RNA 样本，ABclonal Stranded RNA-seq Lib Prep Kit for Illumina 文库产量高宇竞品，且测序数据冗余度低于竞品。
Gene body coverage 分析显示，使用100ng 和 1 μg 的Total RNA 进行文库构建，ABclonal Stranded RNA-seq Lib Prep Kit for Illumina文库转录本覆盖度均一性良好。
不同 Total RNA 投入量中混有相同量的 ERCC mix1标准品（ERCC：ERCC RNA Spike-in Control Mixes， ambion, 4456740）测序结果显示，ABclonal Stranded RNA-seq Lib Prep Kit for Illumina的 ERCC transcript correlation 优于竞品。
Publishing research using RK20349? Please let us know so that we can cite the reference in this datasheet.
