
CUT&Tag was performed using the CUT&Tag Assay Kit (pAG-Tn5) for Illumina (RK20265) from 10⁵ K-562 cells with 1 μg of Acetyl-Histone H3-K9 Rabbit mAb (A21107), followed by incubation with Goat Anti-Rabbit IgG(H+L)(AS070). The CUT&Tag results denote the enrichment pattern of Acetyl-Histone H3-K9 around RPL30 gene.

CUT&Tag was performed using the CUT&Tag Assay Kit (pAG-Tn5) for Illumina (RK20265) from 10⁵ K-562 with 1 μg of Acetyl-Histone H3-K9 Rabbit mAb (A21107), followed by incubation with Goat Anti-Rabbit IgG(H+L)(AS070). The CUT&Tag results denote the enrichment pattern of AAcetyl-Histone H3-K9 Rabbit across chromosome 8 (upper panel) and the genomic region encompassing RPL30, a representative gene enriched in Acetyl-Histone H3-K9 Rabbit (lower panel).

Western blot analysis of various lysates using Acetyl-Histone H3-K9 Rabbit mAb (A21107) at1:1000 dilution. HeLa and NIH/3T3 and C6 cells were treated with TSA (1 uM) at 37℃ for 18 hours.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (RM00020).Exposure time: 10s.

Dot-blot analysis of all sorts of peptides using Acetyl-Histone H3-K9 antibody (A21107) at 1:1000 dilution.

Immunohistochemistry analysis of paraffin-embedded Human colon carcinoma using Acetyl-Histone H3-K9 Rabbit mAb (A21107) at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate buffer (pH 6.0) prior to IHC staining.

Immunohistochemistry analysis of paraffin-embedded Human liver using Acetyl-Histone H3-K9 Rabbit mAb (A21107) at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate buffer (pH 6.0) prior to IHC staining.

Immunohistochemistry analysis of paraffin-embedded Human spleen using Acetyl-Histone H3-K9 Rabbit mAb (A21107) at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate buffer (pH 6.0) prior to IHC staining.

Immunohistochemistry analysis of paraffin-embedded Mouse brain using Acetyl-Histone H3-K9 Rabbit mAb (A21107) at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate buffer (pH 6.0) prior to IHC staining.

Immunohistochemistry analysis of paraffin-embedded Mouse intestin using Acetyl-Histone H3-K9 Rabbit mAb (A21107) at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate buffer (pH 6.0) prior to IHC staining.

Immunohistochemistry analysis of paraffin-embedded Mouse spleen using Acetyl-Histone H3-K9 Rabbit mAb (A21107) at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate buffer (pH 6.0) prior to IHC staining.

Immunohistochemistry analysis of paraffin-embedded Mouse testis using Acetyl-Histone H3-K9 Rabbit mAb (A21107) at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate buffer (pH 6.0) prior to IHC staining.

Immunohistochemistry analysis of paraffin-embedded Rat brain using Acetyl-Histone H3-K9 Rabbit mAb (A21107) at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate buffer (pH 6.0) prior to IHC staining.

Confocal imaging of HeLa TSA and HeLa cells using Acetyl-Histone H3-K9 Rabbit mAb (A21107,dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007,dilution 1:500)(Red).DAPI was used for nuclear staining (Blue). Objective: 100x.

Immunoprecipitation analysis of 600 μg extracts of NIH/3T3 cells using 5 μg Acetyl-Histone H3-K9 antibody (A21107). Western blot was performed from the immunoprecipitate using Acetyl-Histone H3-K9 antibody (A21107) at a dilution of 1:1000.

Chromatin immunoprecipitation analysis of extracts of HeLa cells, using Acetyl-Histone H3-K9 Rabbit mAb (A21107) and rabbit IgG.The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.

Chromatin immunoprecipitation was performed with 15 μg of cross-linked chromatin from 293F cells using 5 μg of Acetyl-Histone H3-K9 Rabbit mAb (A21107). DNA libraries were prepared using Scale ssDNA-seq Lib Prep Kit for Illumina V2 (RK20228). The ChIP sequencing results indicate the enrichment pattern of Acetyl-Histone H3-K9 in the representative genomic region surrounding RPL30 gene.

Chromatin immunoprecipitation was performed with 15 μg of cross-linked chromatin from 293F cells using 5 μg of Acetyl-Histone H3-K9 Rabbit mAb (A21107). DNA libraries were prepared using Scale ssDNA-seq Lib Prep Kit for Illumina V2 (RK20228). The ChIP sequencing results indicate the enrichment pattern of Acetyl-Histone H3-K9 across chromosome 4 (upper panel) and the genomic region encompassing RPL30, a representative gene enriched in Acetyl-Histone H3-K9 (lower panel).