表达宿主
Baculovirus-Insect Cells
纯度
≥ 87 % as determined by SDS-PAGE.
内毒素
< 1.0 EU per μg of the protein as determined by the LAL method
制剂
0.22 μm filtered solution of 20mM PB, 500mM NaCl, pH 6.0, 10% glycerol.
描述
Recombinant Human TLR4/CD284 Protein is produced by Baculovirus-Insect Cells expression system. The target protein is expressed with sequence (Met1-Lys631) of human TLR4/CD284 (Accession #NP_612564.1) fused with a 6×His tag at the C-terminus.
储存
Store at -70℃.This product is stable at ≤ -70°C for up to 1 year from the date of receipt. For optimal storage, aliquot into smaller quantities after centrifugation and store at recommended temperature. Avoid repeated freeze-thaw cycles.未开盖的干粉蛋白在 -20°C至-80°C可保存12个月；
复溶之后，蛋白溶液在-20°C及以下可保存3个月，在2-8℃可保存1周。
生物活性
1.Immobilized Recombinant Human TLR4 / CD284 Protein (His Tag)at 1 μg/mL (100 μl/well) on His Tag Antibody, Mouse MAb precoated (5 μg/mL, 100 μL/well) can bind Anti-TLR4 Antibody, the EC50 is 4-15 ng/mL (QC tested).2.Immobilized Anti-TLR4 Antibody at 2 μg/mL (100 μL/well) can bind Recombinant Human TLR4 / CD284 Protein (His Tag) , the EC50 is 4-15 ng/mL (Routinely tested).
请在蛋白复溶计算器中输入蛋白总质量和所需终浓度，快速计算您需要添加溶液的体积吧！
TLR4, also known as TLR-4, is a member of the Toll-like receptor (TLR) family, which plays a fundamental role in pathogen recognition and activation of innate immunity. TLRs are highly conserved from Drosophila to humans and share structural and functional similarities. They recognize pathogen-associated molecular patterns (PAMPs) that are expressed on infectious agents, and mediate the production of cytokines necessary for the development of effective immunity. TLR4 is most abundantly expressed in placenta, and in myelomonocytic subpopulation of the leukocytes. TLR 4 has also been designated as CD284 (cluster of differentiation 284). It has been implicated in signal transduction events induced by lipopolysaccharide (LPS) found in most gram-negative bacteria. TLR4 Cooperates with LY96 and CD14 to mediate the innate immune response to bacterial lipopolysaccharide (LPS). It acts via MYD88, TIRAP and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. It is also involved in LPS-independent inflammatory responses triggered by Ni(2+).
