A natural gene drive system confers reproductive isolation in rice
Alleviating symptoms of neurodegenerative disorders by astrocyte-specific overexpression of TMEM164 in mice
Application
Plasmid construction and transfection
Programmable RNA base editing with a single gRNA-free enzyme - PMC
Journal
Nucleic acids research
RNF4 controls the extent of replication fork reversal to preserve genome stability
Journal
Nucleic acids research
RNA-binding protein RBM5 plays an essential role in acute myeloid leukemia by activating the oncogenic protein HOXA9 - PMC
Mechanical instability generated by Myosin 19 contributes to mitochondria cristae architecture and OXPHOS
Journal
Nature communications
Application
Plasmid constructions
EGFR core fucosylation, induced by hepatitis C virus, promotes TRIM40-mediated-RIG-I ubiquitination and suppresses interferon-I antiviral defenses - PMC
Journal
Nature communications
LDLR is used as a cell entry receptor by multiple alphaviruses - PMC
Journal
Nature communications
Modular assembly of an artificially concise biocatalytic cascade for the manufacture of phenethylisoquinoline alkaloids - PMC
Journal
Nature communications
ProRefiner: an entropy-based refining strategy for inverse protein folding with global graph attention - PMC
Journal
Nature communications
Unnatural activities and mechanistic insights of cytochrome P450 PikC gained from site-specific mutagenesis by non-canonical amino acids - PMC
Journal
Nature communications
Application
Molecular cloning
Species
Streptomyces venezuelae
A small molecule antagonist of SMN disrupts the interaction between SMN and RNAP II - PMC
Journal
Nature communications
NuRD mediates mitochondrial stress–induced longevity via chromatin remodeling in response to acetyl-CoA level
Application
Restriction Enzyme Digestion
Species
Caenorhabditis elegans
A fitness trade-off between growth and survival governed by Spo0A-mediated proteome allocation constraints in Bacillus subtilis - PMC
Tracking pairwise genomic loci by the ParBParS and Noc-NBS systems in living cells
Journal
Nucleic acids research
A transcription factor-based bacterial biosensor system and its application for on-site detection of explosives
Journal
Biosensors & bioelectronics
Application
Molecular cloning
Maize VKS1 Regulates Mitosis and Cytokinesis during Early Endosperm Development.
Application
Restriction Enzyme Digestion
Combining protein and metabolic engineering strategies for high-level production of L-theanine in Corynebacterium glutamicum
Journal
Bioresource technology
High-level production of D-arabitol by Zygosaccharomyces rouxii from glucose: Metabolic engineering and process optimization
Journal
Bioresource technology
An ULK1/2PXN mechanotransduction pathway suppresses breast cancer cell migration
A widespread single amino acid mutation in AcrA reduces tigecycline susceptibility in Klebsiella pneumoniae
Journal
Microbiology spectrum
Upcycling of PET oligomers from chemical recycling processes to PHA by microbial co-cultivation
Journal
Waste management (New York, N.Y.)
Application
Construction of plasmids and strains
A Novel Mouse Model Harboring Hepatitis B Virus Covalently Closed Circular DNA
Journal
Cell Mol Gastroenterol Hepatol
African Swine Fever Virus Structural Protein p17 Inhibits cGAS-STING Signaling Pathway Through Interacting With STING - PMC
Journal
Frontiers in immunology
Application
Plasmids and Molecular Cloning
Transcription factor OsSNAC1 positively regulates nitrate transporter gene expression in rice
Application
Plasmids and Molecular Cloning
Oxidative stress tolerance contributes to heterologous protein production in Pichia pastoris
Journal
Biotechnol Biofuels
The PRMT5/WDR77 complex restricts hepatitis E virus replication
Application
Plasmid construction
Gly-pro-ala peptide and FGSHF3 exert protective effects in DON-induced toxicity and intestinal damage via decreasing oxidative stress
Journal
Food research international (Ottawa, Ont.)
Application
Seamless cloning
The transcription factor BnaA9.WRKY47 coordinates leaf senescence and nitrogen remobilization in Brassica napus
Journal
Journal of experimental botany
Species
Saccharomyces cerevisiae
MdAIL5 overexpression promotes apple adventitious shoot regeneration by regulating hormone signaling and activating the expression of shoot development-related genes - PMC
Journal
Horticulture research
Application
Construction of vectors and transgenesis
Selective degradation of cellular BRD3 and BRD4-L promoted by PROTAC molecules in six cancer cell lines
Journal
European journal of medicinal chemistry
Construction of Coxsackievirus B5 viruses with luciferase reporters and their applications in vitro and in vivo
Insertion of a TRIM-like sequence in MdFLS2-1 promoter is associated with its allele-specific expression in response to Alternaria alternata in apple - PMC
Journal
Frontiers in plant science
The ATP Synthase Subunits FfATPh, FfATP5, and FfATPb Regulate the Development, Pathogenicity, and Fungicide Sensitivity of Fusarium fujikuroi - PMC
Journal
International journal of molecular sciences
Species
Fusarium fujikuroi
Valorization of Polyethylene Terephthalate to Muconic Acid by Engineering Pseudomonas Putida - PMC
Journal
International journal of molecular sciences
VPS28 regulates brain vasculature by controlling neuronal VEGF trafficking through extracellular vesicle secretion
Application
Plasmid constructions
VPS28 regulates brain vasculature by controlling neuronal VEGF trafficking through extracellular vesicle secretion
Application
Plasmid construction
An Endogenous Retroviral LTR-Derived Long Noncoding RNA lnc-LTR5B Interacts With BiP to Modulate ALV-J Replication in Chicken Cells
Precision Engineering of the Transcription Factor Cre1 in Hypocrea jecorina ( Trichoderma reesei) for Efficient Cellulase Production in the Presence of Glucose
Journal
Front Bioeng Biotechnol
High-level expression of leghemoglobin in Kluyveromyces marxianus by remodeling the heme metabolism pathway - PMC
Journal
Frontiers in bioengineering and biotechnology
Alternative Polyadenylation of Malic Enzyme 1 Is Essential for Accelerated Adipogenesis
Journal
Journal of agricultural and food chemistry
Species
Homo sapiens,Mus musculus
Reprogramming the Metabolic Network in Kluyveromyces lactis with a Transcriptional Switch for De Novo Lacto-N-biose Synthesis
Journal
Journal of agricultural and food chemistry
Species
Kluyveromyces lactis
GPA peptide enhances Nur77 expression in intestinal epithelial cells to exert a 1 protective effect against DSS-induced colitis
Application
Restriction Enzyme Digestion
African Swine Fever Virus A528R Inhibits TLR8 Mediated NF-B Activity by Targeting p65 Activation and Nuclear Translocation
Development of a Genetically Engineered Bivalent Vaccine against Porcine Epidemic Diarrhea Virus and Porcine Rotavirus - PMC
Application
Seamless Cloning
Identification and Characterization of a Novel Quanzhou Mulberry Virus from Mulberry (Morus alba) - PMC
MAVS-Based Reporter Systems for Real-Time Imaging of EV71 Infection and Antiviral Testing - PMC
The First Human Vulvar Intraepithelial Neoplasia Cell Line with Naturally Infected Episomal HPV18 Genome - PMC
Enhanced production of aspochalasin D through genetic engineering of Aspergillus flavipes
Journal
Applied microbiology and biotechnology
Species
Aspergillus flavipes
Alleviating vacuolar transport improves cellulase production in Trichoderma reesei
Journal
Applied microbiology and biotechnology
Species
Trichoderma reesei
Highly efficient biosynthesis of spermidine from L-homoserine and putrescine using an engineered Escherichia coli with NADPH self-sufficient system
Journal
Applied microbiology and biotechnology
The African swine fever virus protease pS273R inhibits DNA sensing cGAS-STING pathway by targeting IKKε
miR319 and its target TCP4 involved in plant architecture regulation in Brassica napus
Journal
Plant science : an international journal of experimental plant biology
Application
Transient expression
Species
Brassica campestris L
Significant Impact of AcrB Amino Acid Polymorphism at Residue 716 on Susceptibility to Tigecycline and Other Antibiotics in Klebsiella pneumoniae
Journal
ACS infectious diseases
Construction and Application of a High-Throughput In Vivo Screening Platform for the Evolution of Nitrile Metabolism-Related Enzymes Based on a Desensitized Repressive Biosensor
Journal
ACS synthetic biology
FfCOX17 is Involved in Fumonisins Production, Growth, Asexual Reproduction, and Fungicide Sensitivity in Fusarium fujikuroi - PMC
Species
Fusarium fujikuroi
Comparative biochemical characterization of mammalian-derived CYP11A1s with cholesterol side-chain cleavage activities
Journal
The Journal of steroid biochemistry and molecular biology
Application
Molecular cloning
The Signal Peptide and Chaperone UNC93B1 Both Influence TLR8 Ectodomain Intracellular Endosomal Localization
Species
Sus scrofa,Bos taurus,Homo sapiens
OGT Binding Peptide-Tagged Strategy Increases Protein O-GlcNAcylation Level in E. coli - PMC
Journal
Molecules (Basel, Switzerland)
Application
Seamless Assembly
Production of D-tagatose, bioethanol, and microbial protein from the dairy industry by-product whey powder using an integrated bioprocess
Journal
Biotechnology journal
An extracellular Cu/Zn superoxide dismutase from Neocaridina denticulata sinensis: cDNA cloning, mRNA expression and characterizations of recombinant protein
Journal
Fish & shellfish immunology
Identification of imidazoquinoline derivative (IQD) interacting sites of porcine TLR8 and the underlying species specificity
High-efficiency genetic engineering toolkit for virus based on lambda red-mediated recombination
Journal
Biotechnology letters
BPIFA2 as a Novel Early Biomarker to Identify Fatal Radiation Injury After Radiation Exposure
Journal
Dose-response : a publication of International Hormesis Society
SRRM2 phase separation drives assembly of nuclear speckle subcompartments
Rapid Visual Detection of African Swine Fever Virus with a CRISPR/Cas12a Lateral Flow Strip Based on Structural Protein Gene D117L - PMC
Application
Cloning of LbCas12a Gene and Protein Expression and Purification
Porcine IKK is involved in the STING induced type I IFN antiviral response of the cytosolic DNA signaling pathway
Journal
The Journal of biological chemistry
Application
Seamless cloning
SARS-CoV-2 main protease cleaves MAGED2 to antagonize host antiviral defense
Application
plasmid construction
Role of cellulose response transporter-like protein CRT2 in cellulase induction in Trichoderma reesei - PMC
Journal
Biotechnology for biofuels and bioproducts
Application
Plasmid construction
Species
Agrobacterium tumefaciens
Purification and functional validation of LtCas12a protein - PMC
A Cas12a ortholog with distinct TTNA PAM enables sensitive detection of HPV16/18 - PMC
Journal
Cell reports methods
Application
Seamless Assembly
Coordination of Polyketide Release and Multiple Detoxification Pathways for Tolerable Production of Fungal Mycotoxins
Journal
Angew Chem Int Ed Engl
Application
Molecular cloning