干细胞培养概述

干细胞指在培养过程中能够多次扩增并保持其多向分化潜能的原代细胞,按发育阶段可分为胚胎干细胞和成体干细胞两大类。胚胎干细胞具有发育的全能性,能分化出成体的所有组织器官。成体干细胞在许多组织和器官的修复、再生过程中起到了关键作用,包括具有分化为血液系统各种细胞潜能的造血干细胞,具有分化为机体骨、软骨和各种器官细胞潜能的间充质干细胞,以及具有分化为神经系统各种细胞潜能的神经干细胞等。不同类型的干细胞能够通过细胞因子改变其增殖、分化或其他特性。

干细胞培养专用细胞因子

诱导多功能干细胞/胚胎干细胞培养常用细胞因子

蛋白名称物种Gene ID标签表达系统货号
基质胶(干细胞培养,无酚红)///RPM0006
Human Pluripotent Stem Cell Medium///RPM0051K
Recombinant Human/Mouse/Rat mature Activin A/INHBA ProteinHuman,Mouse,Rat3624No-TagCHO CellsRP01782S
Recombinant Human/Mouse/Rat mature BMP-2 ProteinHuman,Mouse,Rat650No tagE.coliRP02510S
Recombinant Human/Mouse/Rat Mature BMP-4 ProteinHuman,Mouse,Rat652No-TagCHO cellsRP02512S1
Recombinant Human Mature BMP-7 ProteinHuman655No-TagHEK293 cellsRP02513S
Recombinant Human Dkk-1 ProteinHuman22943C-HisHEK293 cellsRP01343
Recombinant Mouse Dkk-1 ProteinMouse13380C-HisHEK293 cellsRP01062
Recombinant Human EGF ProteinHuman1950No-TagE. coliRP03287
Recombinant Mouse EGF ProteinMouse13645No-tagPichiaRP01684
Recombinant Human FGF-2/bFGF ProteinHuman2247No tagE. coliRP01042
Recombinant Mouse FGF-2/bFGF ProteinMouse14173C-HisE. coliRP01345
Recombinant Rat FGF-2/bFGF ProteinRat54250C-6HisE. coliRP01759
Recombinant Human FGF-3 ProteinHuman2248No tagE. coliRP01404
Recombinant Human FGF-4 ProteinHuman2249NO-tagE. coliRP01712
Recombinant Human FGF-7/HBGF-7/KGF ProteinHuman2252C-6HisHEK293 cellsRP01717
Recombinant Human FGF-9 ProteinHuman2254NO-tagE. coliRP01710
Recombinant Mouse FGF-9 ProteinMouse14180No-tagE. coliRP01840LQ
Recombinant Human FGF-10 ProteinHuman2255No tagE. coliRP01140
Recombinant Mouse FGF-10 ProteinMouse14165NO-TagE. coliRP01848
Recombinant Human FGF-18 ProteinHuman8817C-HisHEK293 cellsRP01827
Recombinant Human FGF-19 ProteinHuman9965C-HisHEK293 cellsRP01231
Recombinant Human Flt3 ligand/Flt3L ProteinHuman2323C-HisHEK293 cellsRP00175
Recombinant Mouse Flt3 ligand/Flt3L ProteinMouse14256C-hFc&HisHEK293 cellsRP01058
Recombinant Human Hepatocyte growth factor/HGF ProteinHuman3082C-HisHEK293 cellsRP01602
Recombinant Mouse Hepatocyte growth factor/HGF ProteinMouse15234C-HisHEK293 cellsRP01584
Recombinant Rat Hepatocyte growth factor/HGF ProteinRat24446C-HisHEK293 cellsRP01776
Recombinant Mouse IGF-I ProteinMouse16000C-hFcHEK293 cellsRP01859
Recombinant Human IL-3 ProteinHuman3562C-HisHEK293 cellsRP01362
Recombinant Mouse IL-3 ProteinMouse16187C-HisHEK293 cellsRP01386
Recombinant Human IL-6 ProteinHuman3569C-HisHEK293 cellsRP00201
Recombinant Mouse IL-6 ProteinMouse16193C-HisHEK293 cellsRP01321
Recombinant Human IL-11 ProteinHuman3589No tagE. coliRP00050
Recombinant Mouse IL-11 ProteinMouse16156No tagE. coliRP02904
Recombinant Human Noggin/NOG ProteinHuman9241C-HisHEK293 cellsRP01237
Recombinant Mouse Noggin/NOG ProteinMouse18121C-HisHEK293 cellsRP01308
Recombinant Human PDGF-BB ProteinHuman5155No-tagPichiaRP01763
Recombinant Mouse PDGF-BB ProteinMouse18591C-6HisHEK293 cellsRP01745
Recombinant Human R-spondin-1/RSPO1 ProteinHuman284654C-HisHEK293 cellsRP00071
Recombinant Mouse R-spondin-1/RSPO1 ProteinMouse192199C-HisHEK293 cellsRP01807
Recombinant Human R-spondin-3/ RSPO3 ProteinHuman84870C-HisHEK293 cellsRP01952
Recombinant Human c-Kit ligand/KITLG/SCF ProteinHuman4254C-HisHEK293 cellsRP00124
Recombinant Mouse c-Kit ligand/KITLG/SCF ProteinMouse17311C-HisHEK293 cellsRP01055
Recombinant Rat c-Kit ligand/KITLG/SCF ProteinRat60427C-HisHEK293 cellsRP01794
Recombinant Human Sonic hedgehog protein N-product/SHH(C24IVI) ProteinHuman6469No tagE. coliRP00056
Recombinant Mouse Sonic hedgehog protein N-product/SHH ProteinMouse20423C-HisHEK293 cellsRP01561
Recombinant Rat Sonic hedgehog N-product/SHH ProteinRat29499C-HisHEK293 cellsRP01896
Recombinant Human Mature TGF-beta 1 ProteinHuman7040No tagHEK293 cellsRP01458
Recombinant Mouse/Rat Mature TGF-beta 1 ProteinMouse21803No tagHEK293 cellsRP00671
Recombinant Human Thrombopoietin/THPO ProteinHuman7066C-HisHEK293 cellsRP00174
Recombinant Mouse Thrombopoietin/THPO ProteinMouse21832C-6HisHEK293 cellsRP01665
Recombinant Rat Thrombopoietin /THPO / TPO ProteinRat81811C-HisHEK293 cellsRP01901
Recombinant Human VEGF-A/VEGF165 ProteinHuman7422N-HisHEK293 cellsRP01150
Recombinant Human Wnt-3a Surrogate ProteinHuman/C-hFcHEK293 CellsRP01618SLQ
Recombinant Human DLL4 ProteinHuman54567C-HisHEK293 cellsRP00356
Recombinant Mouse DLL4 ProteinMouse54485C-6HisHEK293 cellsRP01681
Recombinant Human VCAM-1/CD106 ProteinHuman7412C-HisHEK293 cellsRP00972
Recombinant Mouse VCAM-1/CD106 ProteinMouse22329C-hFc&HisHEK293 cellsRP01227

胚胎/诱导多能干细胞(ESC/iPSC)培养验证数据展示

部分竞品对比数据展示
Recombinant Human/Mouse/Rat mature Activin A/INHBA inhibit proliferation of MPC-11 cells
Recombinant Human/Mouse/Rat mature BMP-4 Protein induce alkaline phosphatase production by ATDC5 mouse chondrogenic cells
Recombinant Human Leukemia inhibitory factor/LIF stimulates cell proliferation of the TF-1 cells
客户培养数据展示
浙江大学
Experiment Type
Cell-based assay
Sample
iPSC
Product
Recombinant Human/Mouse/Rat mature Activin A/INHBA Protein (RP01782S) (Upgraded product)
Recombinant Human/Mouse/Rat Mature BMP-4 Protein (RP02512S1) (Upgraded product)
Recombinant Human VEGF-A/VEGF121 Protein (RP01162)
Recombinant Human FGF-2/bFGF Protein (RP01042)
Recombinant Human Thrombopoietin/THPO Protein (RP00174)
Recombinant Human IL-3 Protein (RP01362)
Recombinant Human IL-6 Protein (RP00201)
Recombinant Human IL-11 Protein (RP00050)
Description
A. Recombinant Activin A (50 ng/mL, Cat. RP01782S) and BMP-4 (40 ng/mL, Cat. RP02512S1) were used to induce iPSC into mesodermal cells differentiation. After 4 days of induction, the proportion of cobblestone-like cells expressed CD43+ reached 50.7%.
B. Recombinant VEGF (40 ng/mL, Cat. RP01162) and bFGF (50 ng/mL, Cat. RP01042) were used to induce mesodermal cells differentiation into HSPCs. After 4 days of induction, the proportion of cobblestone-like cells expressed CD43+ reached 42.5%.
C. Recombinant TPO (50 ng/mL, Cat. RP00174), IL-3 (15 ng/mL, Cat. RP01362), IL-6 (15 ng/mL, Cat. RP00201) and IL-11 (15 ng/mL, Cat. RP00050) were used to induce HSPCs differentiation into megakaryocytes. After 6 days of induction, the proportion of cells expressed CD41/42+ reached 41%.

间充质干细胞培养常用细胞因子

蛋白名称物种Gene ID标签表达系统货号
Recombinant Human/Mouse/Rat BDNF ProteinHuman,Mouse,Rat627C-HisE. coliRP01243
Recombinant Human/Mouse/Rat mature BMP-2 ProteinHuman,Mouse,Rat650No tagE.coliRP02510S
Recombinant Human/Mouse/Rat Mature BMP-4 ProteinHuman,Mouse,Rat652No-TagCHO cellsRP02512S1
Recombinant Human CNTF ProteinHuman1270C-HisE. coliRP00039
Recombinant Rat CNTF ProteinRat25707C-6HisE. coliRP01671
Recombinant Human EGF ProteinHuman1950No-TagE. coliRP03287
Recombinant Human FGF-2/bFGF ProteinHuman2247No tagE. coliRP01042
Recombinant Mouse FGF-2/bFGF ProteinMouse14173C-HisE. coliRP01345
Recombinant Rat FGF-2/bFGF ProteinRat54250C-6HisE. coliRP01759
Recombinant Human FGF-4 ProteinHuman2249NO-tagE. coliRP01712
Recombinant Human/Mouse FGF-8B ProteinHuman/Mouse2253(B)C-6HisHEK293 cellsRP01659
Recombinant Human FGF-10 ProteinHuman2255No tagE. coliRP01140
Recombinant Mouse FGF-10 ProteinMouse14165NO-TagE. coliRP01848
Recombinant Mouse IGF-I ProteinMouse16000C-hFcHEK293 cellsRP01859
Recombinant Human Beta-nerve growth factor/NGFB ProteinHuman4803C-HisHEK293 cellsRP01792
Recombinant Mouse Mature Beta-nerve growth factor/NGFB ProteinMouse18049No tagE. coliRP00667
Recombinant Human Noggin/NOG ProteinHuman9241C-HisHEK293 cellsRP01237
Recombinant Mouse Noggin/NOG ProteinMouse18121C-HisHEK293 cellsRP01308
Recombinant Human PDGF-BB ProteinHuman5155No-tagPichiaRP01763
Recombinant Mouse PDGF-BB ProteinMouse18591C-6HisHEK293 cellsRP01745
Recombinant Human Sonic hedgehog protein N-product/SHH(C24IVI) ProteinHuman6469No tagE. coliRP00056
Recombinant Mouse Sonic hedgehog protein N-product/SHH ProteinMouse20423C-HisHEK293 cellsRP01561
Recombinant Rat Sonic hedgehog N-product/SHH ProteinRat29499C-HisHEK293 cellsRP01896
Recombinant Human VEGF-A/VEGF165 ProteinHuman7422N-HisHEK293 cellsRP01150

间充质干细胞培养验证数据展示

部分竞品对比数据展示
Recombinant Human/Mouse/Rat mature BMP-2 Protein induce alkaline phosphatase production by ATDC5 mouse chondrogenic cells
Recombinant Human/Mouse/Rat mature BMP-4 Protein induce alkaline phosphatase production by ATDC5 mouse chondrogenic cells
Recombinant Human Mature TGF-beta 1 inhibit proliferation of HT‑2 mouse T cells
客户培养数据展示
厦门大学
Experiment Type
Cell-based assay
Sample
Dermal papillary
Product
Recombinant Mouse FGF-2/bFGF Protein (RP01345)
Description
Recombinant mouse FGF-2 (1 ng/mL, Cat. RP01345) was used to culture dermal papillary (DP) cells. The immunofluorescence identification showed that FGF-2 could maintain the cellular characteristics of DP cells. Note: The red part is fluorescence-labelled PDFGRA antibody, and blue/cyan part is the DAPI-stained cells.

神经干细胞培养常用细胞因子

蛋白名称物种Gene ID标签表达系统货号
Recombinant Human/Mouse/Rat mature BMP-2 ProteinHuman,Mouse,Rat650No tagE.coliRP02510S
Recombinant Human/Mouse/Rat Mature BMP-4 ProteinHuman,Mouse,Rat652No-TagCHO cellsRP02512S1
Recombinant Human EGF ProteinHuman1950No-TagE. coliRP03287
Recombinant Human FGF-2/bFGF ProteinHuman2247No tagE. coliRP01042
Recombinant Mouse FGF-2/bFGF ProteinMouse14173C-HisE. coliRP01345
Recombinant Rat FGF-2/bFGF ProteinRat54250C-6HisE. coliRP01759
Recombinant Human FGF-4 ProteinHuman2249NO-tagE. coliRP01712
Recombinant Human HB-EGF ProteinHuman1839C-HisHEK293 cellsRP00754
Recombinant Mouse IGF-I ProteinMouse16000C-hFcHEK293 cellsRP01859
Recombinant Human IL-6 ProteinHuman3569C-HisHEK293 cellsRP00201
Recombinant Mouse IL-6 ProteinMouse16193C-HisHEK293 cellsRP01321
Recombinant Human PDGF-BB ProteinHuman5155No-tagPichiaRP01763
Recombinant Mouse PDGF-BB ProteinMouse18591C-6HisHEK293 cellsRP01745
Recombinant Human Sonic hedgehog protein N-product/SHH(C24IVI) ProteinHuman6469No tagE. coliRP00056
Recombinant Mouse Sonic hedgehog protein N-product/SHH ProteinMouse20423C-HisHEK293 cellsRP01561
Recombinant Rat Sonic hedgehog N-product/SHH ProteinRat29499C-HisHEK293 cellsRP01896
Recombinant Human Mature TGF-beta 1 ProteinHuman7040No tagHEK293 cellsRP01458
Recombinant Mouse/Rat Mature TGF-beta 1 ProteinMouse21803No tagHEK293 cellsRP00671
Recombinant Human Mature TGF-beta 2 ProteinHuman7042No tagHEK293 cellsRP00452
Recombinant Mouse/Rat Mature TGF-beta 2 ProteinMouse/Rat21808No tagHEK293 cellsRP00672
Recombinant Human/Mouse/Rat mature TGF-beta 3 ProteinHuman7043No tagHEK293 cellsRP00645
Recombinant Human Oncostatin-M/OSM ProteinHuman5008No tagE. coliRP00054
Recombinant Mouse Oncostatin-M/OSM ProteinMouse18413C-HisHEK293 cellsRP01639
Recombinant Rat Oncostatin-M/OSM ProteinRat289747C-HisHEK293 cellsRP01844

神经干细胞培养验证数据展示

部分竞品对比数据展示
Measured by its binding ability in a functional ELISA.Immobilized Recombinant Human TrkB (Catalog: RP00290) at 2μg/mL (100 μL/well) can bind Recombinant Human Neurotrophin-3/NGF-2/NT-3 with a linear range of 1.95-35.5 ng/mL.
Recombinant Human CNTF stimulates cell proliferation of the TF-1 human erythroleukemic cell line.
客户培养数据展示
南京鼓楼医院
Experiment Type
Cell-based assay
Sample
Primary neural stem cells
Product
Recombinant Human FGF-2/bFGF Protein (RP01042)
Description
Primary neural stem cells were cultured with a final concentration of 20 ng/mL FGF2 (Cat. RP01042), and as shown in the figure, the size of the suspended neural stem cells gradually increased