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Development of an Enzyme-Linked Immunosorbent Assay (ELISA) for Accurate and Prompt Coronavirus Disease 2019 (COVID-19) Diagnosis Using the Rational Selection of Serological Biomarkers
Recombinant SARS-CoV-2 Nucleocapsid(G335A) Protein is produced by Baculovirus-Infected Sf9 Cells expression system. The target protein is expressed with sequence (Ser2-Ala419（Gly335Ala）) of sars-cov-2 Nucleocapsid (Accession #QHD43423.2) fused with a 6×His tag at the N-terminus.
标签
N-His
纯度
> 90% by SDS-PAGE.
内毒素
< 0.1 EU/μg of the protein by LAL method.
生物活性
1.Measured by its binding ability in a functional ELISA. Immobilized Recombinant SARS-CoV-2 Nucleocapsid Protein at 2 μg/mL (100 μL/well) can bind SARS-CoV-2 N Protein Rabbit mAb with a linear range of 0.032-0.79 ng/mL. 2.Measured by its binding ability in a functional ELISA. Immobilized Recombinant SARS-CoV-2 envelope Protein at 2 μg/mL (100 μL/well) can bind Recombinant SARS-CoV-2 Nucleocapsid protein, the EC50 of Recombinant SARS-CoV-2 Nucleocapsid protein is 46.90 ng/mL.
制剂
Supplied as a 0.22 μm filtered solution in 20mM Tris, 500mM NaCl, 0.1mM EDTA, 10% glycerol, pH8.0.Contact us for customized product form or formulation.
储存
This liquid product is stable at ≤ ‑70°C for up to 1 year from the date of receipt. For optimal storage, aliquot into smaller quantities after centrifugation and store at recommended temperature.未开盖的干粉蛋白在 -20°C至-80°C可保存12个月； 复溶之后，蛋白溶液在-20°C及以下可保存3个月，在2-8℃可保存1周。
