Recombinant Mouse TNFSF11/RANKL/CD254 Protein determined by SDS-PAGE with Coomassie Blue. showing a band at 15-25 kDa.
Measured by its ability to induce osteoclast differentiation of RAW 264.7 mouse monocyte/macrophage cells. The ED50 for this effect is 2.30-9.22 ng/mL, corresponding to a specific activity of 1.08×105~4.35×105 units/mg.
Induce mouse BMMs to differentiate into osteoclasts with recombinant mouse RANKL (50-400 ng/mL) and M-CSF (25 ng/mL, Cat. RP01216). Replace with fresh medium every two days. After 5 days' stimulation, the cells were fixed, and TRAP staining was performed. Results showed that BMMs differentiated into osteoclasts successfully. (Customer feedback data)
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Mouse tumor necrosis factor ligand superfamily member 11(Tnfsf11) is a member of the tumor necrosis factor(TNF) cytokine family. Tnfsf11 is widely expressed in cells including T cells and T cell rich organs, such asthymus and lymph nodes. This cytokine can bind to TNFRSF11B/OPG andTNFRSF11A/RANK. Tnfsf11 is involvedin a number of fundamental biological processes such as acting as regulator of interactions between T-cells anddendritic cells, the regulation of the T-cell-dependent immune response and enhancing bone-resorption inhumoral hypercalcemia of malignancy. It augments the ability of dendritic cells to stimulate naive T-cellproliferation.
