Recombinant Human S100-A12 Protein was determined by SDS-PAGE with Coomassie Blue, showing a band at approximately 10 kDa.
Immobilized Recombinant Human S100A12 at 2 μg/mL (100 μL/well) can bind recombinant Human AGER/RAGE with a linear range of 0.1-27.25 ng/mL.
The protein is a member of the S100 family of proteins containing 2 EF-hand calcium-binding motifs. S100 proteins are localized in the cytoplasm and nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. S100 proteins include at least 13 members which are located as a cluster on chromosome 1q21. This protein is proposed to be involved in specific calcium-dependent signal transduction pathways and its regulatory effect on cytoskeletal components may modulate various neutrophil activities. The protein includes an antimicrobial peptide which has antibacterial activity.
