骨细胞培养概述

骨骼系统作为支持和保护身体内部器官的结构框架,主要由软骨和骨骼组成。骨骼生长和发育的过程中涉及到许多细胞类型,包括来源于骨髓间充质干细胞的成骨细胞和软骨细胞,以及来源于造血干细胞的破骨细胞,这些过程都离不开各类细胞因子的参与。

骨细胞培养专用细胞因子

破骨细胞培养常用细胞因子

蛋白名称物种Gene ID标签表达系统货号
Recombinant Human TNFSF11/RANKL/CD254 ProteinHuman8600N-HisHEK293 cellsRP00183
Recombinant Mouse TNFSF11/RANKL/CD254 ProteinMouse21943N-His&N-hFCHEK293 cellsRP02134
Recombinant Human CSF-1/M-CSF ProteinHuman1435C-HisHEK293 cellsRP01221
Recombinant Mouse CSF-1/M-CSF ProteinMouse12977No-TagHEK293 cellsRP01216S
Recombinant Rat CSF-1/M-CSF ProteinRat78965C-HisHEK293 cellsRP01847
TRAP 染色试剂盒///RPM0052K

破骨细胞培养验证数据展示

部分竞品对比数据展示
Recombinant Mouse CSF-1/M-CSF stimulates cell proliferation of the M-NFS-60 cells.
M-CSF (50 ng/mL) and RANKL (100 ng/mL) induced differentiation of osteoclasts from primary mouse bone marrow cells for 3 days(top);RANKL (100 ng/mL) induced differentiation of osteoclasts from RAW264.7 cells for 5 days (below).
客户培养数据展示
中国人民解放军陆军军医大学第一附属医院
Experiment Type
Cell-based assay
Sample
Mouse bone marrow cells
Product
Recombinant Mouse CSF-1/M-CSF Protein (RP01216S)
Recombinant Mouse TNFSF11/RANKL/CD254 Protein (RP02134)
Description
Osteoclast induced from primary mouse bone marrow cells

成骨细胞培养常用细胞因子

成骨细胞培养验证数据展示

部分竞品对比数据展示
Recombinant Human/Mouse/Rat mature BMP-2 Protein induce alkaline phosphatase production by ATDC5 mouse chondrogenic cells
Recombinant Human/Mouse/Rat mature BMP-4 Protein induce alkaline phosphatase production by ATDC5 mouse chondrogenic cells
Recombinant Human Mature TGF-beta 1 inhibit proliferation of HT‑2 mouse T cells
客户培养数据展示
华中科技大学同济医学院附属协和医院
Experiment Type
Cell-based assay
Sample
MC3T3
Product
Recombinant Human/Mouse/Rat mature BMP-2 Protein (RP02510S)
Description
Hydrogel-loaded BMP-2 (Cat.RP02510S) promoted the osteogenic differentiation of mouse embryonic osteoblastic precursor cell line MC3T3. After 7 days, ALP staining was used to observe the cell differentiation, and the effect was significant.

软骨细胞培养常用细胞因子

软骨细胞培养验证数据展示

部分竞品对比数据展示
客户培养数据展示
武汉大学口腔医院
Experiment Type
Cell-based assay
Sample
ESC-H9
Product
Recombinant Human/Mouse/Rat mature TGF-beta 3 Protein (RP00645)
Description
After mesenchymal differentiation of ESC-H9 cells was induced, a special medium containing 20ng/ml TGF-β3 was added to induce their differentiation into chondrocytes for 14 days. Microscopic observation revealed that the differentiation rate of chondrocytes was verified to reach about 80%, and the cytokine effect was excellent.
南京鼓楼医院
Experiment Type
Cell-based assay
Sample
Chondrocytes
Product
Recombinant Human IL-1 beta Protein (RP00002)
Description
Human chondrocytes treated with 25 ng/mL IL-1β for 24 h showed increased expressions of type I collagen (COL I) and type X collagen (COL X), and decreased expressions of type II collagen (COL II) and glycosaminoglycan (ACAN), as expected.