骨细胞培养概述

骨骼系统作为支持和保护身体内部器官的结构框架，主要由软骨和骨骼组成。骨骼生长和发育的过程中涉及到许多细胞类型，包括来源于骨髓间充质干细胞的成骨细胞和软骨细胞，以及来源于造血干细胞的破骨细胞，这些过程都离不开各类细胞因子的参与。

骨细胞培养专用细胞因子

破骨细胞培养常用细胞因子

蛋白名称物种Gene ID标签表达系统货号
Recombinant Human TNFSF11/RANKL/CD254 ProteinHuman8600N-HisHEK293 cellsRP00183
Recombinant Mouse TNFSF11/RANKL/CD254 ProteinMouse21943N-His＆N-hFCHEK293 cellsRP02134
Recombinant Human CSF-1/M-CSF ProteinHuman1435C-HisHEK293 cellsRP01221
Recombinant Mouse CSF-1/M-CSF ProteinMouse12977No-TagHEK293 cellsRP01216S
Recombinant Rat CSF-1/M-CSF ProteinRat78965C-HisHEK293 cellsRP01847
TRAP 染色试剂盒///RPM0052K

破骨细胞培养验证数据展示

部分竞品对比数据展示

RP01216S/RP01216

Mouse M-CSF Protein

Recombinant Mouse CSF-1/M-CSF stimulates cell proliferation of the M-NFS-60 cells.

RP01216S/RP02134

Mouse M-CSF Protein, Mouse TNFSF11/RANKL/CD254 Protein

M-CSF (50 ng/mL) and RANKL (100 ng/mL) induced differentiation of osteoclasts from primary mouse bone marrow cells for 3 days(top);RANKL (100 ng/mL) induced differentiation of osteoclasts from RAW264.7 cells for 5 days (below).
客户培养数据展示
中国人民解放军陆军军医大学第一附属医院
Experiment Type
Cell-based assay
Sample
Mouse bone marrow cells
Product
Recombinant Mouse CSF-1/M-CSF Protein (RP01216S)
Recombinant Mouse TNFSF11/RANKL/CD254 Protein (RP02134)
Description
Osteoclast induced from primary mouse bone marrow cells

成骨细胞培养常用细胞因子

蛋白名称物种Gene ID标签表达系统货号
Recombinant Human/Mouse/Rat mature BMP-2 ProteinHuman,Mouse,Rat650No tagE.coliRP02510S
Recombinant Human/Mouse/Rat Mature BMP-4 ProteinHuman,Mouse,Rat652No-TagCHO cellsRP02512S1
Recombinant Human FGF-2/bFGF ProteinHuman2247No tagE. coliRP01042
Recombinant Mouse FGF-2/bFGF ProteinMouse14173C-HisE. coliRP01345
Recombinant Rat FGF-2/bFGF ProteinRat54250C-6HisE. coliRP01759
Recombinant Mouse IGF-I ProteinMouse16000C-hFcHEK293 cellsRP01859
Recombinant Human Mature TGF-beta 1 ProteinHuman7040No tagHEK293 cellsRP01458
Recombinant Mouse/Rat Mature TGF-beta 1 ProteinMouse21803No tagHEK293 cellsRP00671
Recombinant Human Mature TGF-beta 2 ProteinHuman7042No tagHEK293 cellsRP00452
Recombinant Mouse/Rat Mature TGF-beta 2 ProteinMouse/Rat21808No tagHEK293 cellsRP00672
Recombinant Human/Mouse/Rat mature TGF-beta 3 ProteinHuman7043No tagHEK293 cellsRP00645
Recombinant Human Parathormone/PTH ProteinHuman5741No tagE. coliRP01587
Recombinant Mouse Parathormone/PTH ProteinMouse19226No tagE. coliRP01578
Recombinant Human Leukemia inhibitory factor/LIF ProteinHuman3976C-HisHEK293 cellsRP00089
Recombinant Mouse Leukemia inhibitory factor/LIF ProteinMouse16878C-His&AviHEK293 cellsRP01416
Recombinant Rat Leukemia inhibitory factor/LIF ProteinRat60584C-HisHEK293 cellsRP01892

成骨细胞培养验证数据展示

部分竞品对比数据展示

RP02510S

Human/Mouse/Rat BMP-2 Protein

Recombinant Human/Mouse/Rat mature BMP-2 Protein induce alkaline phosphatase production by ATDC5 mouse chondrogenic cells

RP02512S1

Human/Mouse/Rat BMP-4 Protein

Recombinant Human/Mouse/Rat mature BMP-4 Protein induce alkaline phosphatase production by ATDC5 mouse chondrogenic cells

RP01458

Human Mature TGF-beta 1 Protein

Recombinant Human Mature TGF-beta 1 inhibit proliferation of HT‑2 mouse T cells
客户培养数据展示
华中科技大学同济医学院附属协和医院
Experiment Type
Cell-based assay
Sample
MC3T3
Product
Recombinant Human/Mouse/Rat mature BMP-2 Protein (RP02510S)
Description
Hydrogel-loaded BMP-2 (Cat.RP02510S) promoted the osteogenic differentiation of mouse embryonic osteoblastic precursor cell line MC3T3. After 7 days, ALP staining was used to observe the cell differentiation, and the effect was significant.

软骨细胞培养常用细胞因子

蛋白名称物种Gene ID标签表达系统货号
Recombinant Human FGF-2/bFGF ProteinHuman2247No tagE. coliRP01042
Recombinant Mouse FGF-2/bFGF ProteinMouse14173C-HisE. coliRP01345
Recombinant Rat FGF-2/bFGF ProteinRat54250C-6HisE. coliRP01759
Recombinant Human FGF-4 ProteinHuman2249NO-tagE. coliRP01712
Recombinant Human/Mouse FGF-8B ProteinHuman/Mouse2253（B)C-6HisHEK293 cellsRP01659
Recombinant Human FGF-9 ProteinHuman2254NO-tagE. coliRP01710
Recombinant Mouse FGF-9 ProteinMouse14180No-tagE. coliRP01840LQ
Recombinant Human FGF-10 ProteinHuman2255No tagE. coliRP01140
Recombinant Mouse FGF-10 ProteinMouse14165NO-TagE. coliRP01848
Recombinant Mouse IGF-I ProteinMouse16000C-hFcHEK293 cellsRP01859
Recombinant Human Mature TGF-beta 1 ProteinHuman7040No tagHEK293 cellsRP01458
Recombinant Mouse/Rat Mature TGF-beta 1 ProteinMouse21803No tagHEK293 cellsRP00671
Recombinant Human Mature TGF-beta 2 ProteinHuman7042No tagHEK293 cellsRP00452
Recombinant Mouse/Rat Mature TGF-beta 2 ProteinMouse/Rat21808No tagHEK293 cellsRP00672
Recombinant Human/Mouse/Rat mature TGF-beta 3 ProteinHuman7043No tagHEK293 cellsRP00645
Recombinant Human VEGF-A/VEGF165 ProteinHuman7422N-HisHEK293 cellsRP01150
Recombinant Human VEGF-A/VEGF121 ProteinHuman7422N-HisHEK293 cellsRP01162
Recombinant Mouse VEGF-A/VEGF164 ProteinMouse22339N-HisHEK293 cellsRP01060
Recombinant Rat VEGF-A/VEGF164 ProteinRat83785C-HisHEK293 cellsRP01323
Recombinant Human IL-8/CXCL8/GCP-1 ProteinHuman3576No tagE. coliRP00052

软骨细胞培养验证数据展示

部分竞品对比数据展示
客户培养数据展示
武汉大学口腔医院
Experiment Type
Cell-based assay
Sample
ESC-H9
Product
Recombinant Human/Mouse/Rat mature TGF-beta 3 Protein (RP00645)
Description
After mesenchymal differentiation of ESC-H9 cells was induced, a special medium containing 20ng/ml TGF-β3 was added to induce their differentiation into chondrocytes for 14 days. Microscopic observation revealed that the differentiation rate of chondrocytes was verified to reach about 80%, and the cytokine effect was excellent.
南京鼓楼医院
Experiment Type
Cell-based assay
Sample
Chondrocytes
Product
Recombinant Human IL-1 beta Protein (RP00002)
Description
Human chondrocytes treated with 25 ng/mL IL-1β for 24 h showed increased expressions of type I collagen (COL I) and type X collagen (COL X), and decreased expressions of type II collagen (COL II) and glycosaminoglycan (ACAN), as expected.