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Recombinant Human FGFR-2 alpha (IIIb)/KGFR/CD332 Protein (RP02530)

Human FGFR2 alpha (IIIb) on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

The purity of Human FGFR2 alpha (IIIb) is greater than 95% as determined by SEC-HPLC.

Immobilized Human FGFR2 alpha IIIb, hFc tag at 0.5μg/ml (100μl/Well). Dose response curve for Biotinylated Anti-FGFR2 Ab., hFc Tag with the EC50 of 10.0ng/ml determined by ELISA.

All(3)|
货号: RP02530
促销价:   ¥3200
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详细信息

种属
Human
表达宿主
HEK293 cells
Calculated MW
66.4 kDa
Observed MW
80-115 kDa
标签
C-hFc
纯度
> 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
内毒素
Less than 1EU per μg by the LAL method.
描述
Recombinant Human FGFR-2 alpha (IIIb)/KGFR/CD332 Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Arg22-Glu378) of human FGFR-2 alpha (IIIb)/KGFR/CD332 (Accession #P21802-3) fused with hFc tag at the C-terminus.
储存
Store at -20℃.Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt.
After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week.未开盖的干粉蛋白在 -20°C至-80°C可保存12个月;
复溶之后,蛋白溶液在-20°C及以下可保存3个月,在2-8℃可保存1周。
复溶
Centrifuge the tube before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.收到重组蛋白产品之后请检查蛋白冻干粉末是否贴于瓶底,如果粉末浮起,开盖之前请先低温离心。将蛋白用说明书中指定的缓冲液复溶至0.1-0.5 mg/mL(请注意蛋白复溶浓度不能低于0.1 mg/mL),室温平衡5-10 min保证充分溶解,复溶过程中请不要剧烈涡旋及吹打蛋白溶液。如需长期储存,建议复溶时添加载体蛋白或者稳定剂(如0.1% BSA, 5% HSA, 10% FBS 或者 5% 海藻糖),同时将复溶后的蛋白溶液按照需求进行分装,储存于-20°C至-80°C,随取随用,避免反复冻融。

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请在蛋白复溶计算器中输入蛋白总质量和所需终浓度,快速计算您需要添加溶液的体积吧!
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背景信息

Four distinct genes encoding closely related FGF receptors, FGF R1 - 4, are known. All four genes for FGF Rs encode proteins with an N-terminal signal peptide, three immunoglobulin (Ig)-like domains, an acid-box region containing a run of acidic residues between the IgI and IgII domains, a transmembrane domain and the split tyrosine-kinase domain. Multiple forms of FGF R1 - 3 are generated by alternative splicing of the mRNAs. A frequent splicing event involving FGF R1 and 2 results in receptors containing all three Ig domains, referred to as the alpha? isoform, or only IgII and IgIII, referred to as the beta? isoform.

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