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活性验证

Recombinant Human Trk-A/NTRK1 Protein (RP00088)

Recombinant Human Trk-A/NTRK1 Protein was determined by SDS-PAGE with Coomassie Blue, showing a band at 110-120 kDa..

Recombinant Human TrkA inhibit NGF-induced proliferation of TF-1 human erythroleukemic cells. The ED50 for this effect is typically 12-48 ng/mL in the presence of 10 ng/ml of recombinant human NGF.

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货号: RP00088
促销价:   ¥620
货    期:现货产品
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详细信息

种属
Human
表达宿主
HEK293 cells
Calculated MW
68.30 kDa
Observed MW
110-120 kDa
标签
C-hFc&His
纯度
> 97% by SDS-PAGE.
内毒素
< 0.1 EU/μg of the protein by LAL method.
制剂
Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.Contact us for customized product form or formulation.
描述
Recombinant Human Trk-A/NTRK1 Protein is produced by HEK293 expression system. The target protein is expressed with sequence (Ala33-Glu407) of human TrkA (Accession #NP_001012331.1) fused with an Fc, 6×His tag at the C-terminus.
储存
Store at -20℃.Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt.
After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week.未开盖的干粉蛋白在 -20°C至-80°C可保存12个月;
复溶之后,蛋白溶液在-20°C及以下可保存3个月,在2-8℃可保存1周。
生物活性
Measured by its ability to inhibit NGF-induced proliferation of TF-1 human erythroleukemic cells. The ED50 for this effect is typically 12-48 ng/mL in the presence of 10 ng/mL of recombinant human NGF.
复溶
Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.收到重组蛋白产品之后请检查蛋白冻干粉末是否贴于瓶底,如果粉末浮起,开盖之前请先低温离心。将蛋白用说明书中指定的缓冲液复溶至0.1-0.5 mg/mL(请注意蛋白复溶浓度不能低于0.1 mg/mL),室温平衡5-10 min保证充分溶解,复溶过程中请不要剧烈涡旋及吹打蛋白溶液。如需长期储存,建议复溶时添加载体蛋白或者稳定剂(如0.1% BSA, 5% HSA, 10% FBS 或者 5% 海藻糖),同时将复溶后的蛋白溶液按照需求进行分装,储存于-20°C至-80°C,随取随用,避免反复冻融。

蛋白复溶计算器

请在蛋白复溶计算器中输入蛋白总质量和所需终浓度,快速计算您需要添加溶液的体积吧!
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背景信息

This protein is a member of the neurotrophic tyrosine kinase receptor (NTKR) family. This kinase is a membrane-bound receptor that, upon neurotrophin binding, phosphorylates itself and members of the MAPK pathway. The presence of this kinase leads to cell differentiation and may play a role in specifying sensory neuron subtypes. Mutations in this gene have been associated with congenital insensitivity to pain, anhidrosis, self-mutilating behavior, mental retardation and cancer. Alternate transcriptional splice variants of this gene have been found, but only three have been characterized to date.

基因ID
Swiss Prot
别名
MTC; TRK; TRK1; TRKA; Trk-A; p140-TrkA;NTRK1;TRK;TRK1;TRKA;Trk-A;p140-TrkA
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