产品中心 / 产品类型 / 细胞系&裂解液 / KO细胞裂解液

CTNNA1 Knockout 293T Cell Lysate, Homozygous (RM02039)

说明书 联系我们 收藏

Genome sequence analysis of PCR products from parental (WT) and CTNNA1 knockout (KO) 293T cells, using sanger sequencing.

All(1)|
货号: RM02039
促销价：   ￥4700
抗体定制服务咨询

文献引用 (0)

详细信息

靶点
CTNNA1
细胞系
293T
突变描述
CTNNA1 Knockout 293T Cell Line is engineered from 293T cell line with Gene-Editing technology.
Allele-1:85bp deletion in exon2
Allele-2:85bp deletion in exon2

Mammalian cells such as human, rat and mouse cells are normally diploid with two alleles.
Homozygote: both alleles were knocked out, mRNA has no signal, no expression of proteins.
Heterozygote: only one allele was knocked out, the mRNA transcript levels was decreased compared to wild type, and the protein expression levels was also lower than that of the wild type.
敲除验证
Sanger Sequencing
产品组成
1 vial parental cell Lysate and 1 vial knockout cell Lysate
裂解液量
50μL, 2μg/μL.
使用方法
To be used as WB control. Lysate is supplied in 1× SDS sample buffer (2% SDS, 60 mM
Tris-HCl pH 6.8, 10% Glycerol, 0.02% Bromophenol blue, 60 mM beta-mercaptoethanol).
Lysate should be boiled for 3 - 5 minutes before loading onto gel.
物种
Human
保存条件
Lysate is stable for 12 months when stored at -20℃. Minimizing freeze-thaw cycles.
运输条件
4℃

背景信息

This gene encodes a member of the catenin family of proteins that play an important role in cell adhesion process by connecting cadherins located on the plasma membrane to the actin filaments inside the cell. The encoded mechanosensing protein contains three vinculin homology domains and undergoes conformational changes in response to cytoskeletal tension, resulting in the reconfiguration of cadherin-actin filament connections. Certain mutations in this gene cause butterfly-shaped pigment dystrophy. [provided by RefSeq, May 2016]

基因ID
1495
Swiss Prot
P35221
别名
CAP102; MDPT2
研究领域
>