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Multicolor flow cytometry panel to identify mouse T cells, NK cells. Mouse splencytes were stained with anti-mouse CD3e FITC, CD4 ABflo® 647, CD8a ABflo® 594, NK1.1 PE. All antibodies are validated for cytometry. CD4 T cells are defined as CD3e+ CD4+; CD8 T cells are defined as CD3e+ CD8a+; NK cells are CD3- NK1.1+.
The 4-fluorescence Mouse T/NK panel has been designed for reliable identification of mouse T and NK lymphocyte populations in mouse blood or tissue samples.
ABflo® multi-fluorescence panel RK40003 covers the core CD markers of CD3e, CD4, CD8a, and CD161c/NK1.1. Our plots showed clear subgroups of CD3e+ T cells, CD4+ Th cells, CD8a+ CTL cells, and CD161c/NK1.1+ NK cells (cite figures).
| Target | Catalog No. | Fluorochrome | Ex/Em (nm) | Isotype | Clone | Size | Vol. Per Test |
| CD3e | A23322 | FITC | 491/516 | Armenian Hamster IgG | ACC0001 | 100 μg | 1 μL |
| CD4 | A24721 | ABflo® 647 | 648/664 | Rabbit IgG | ARC64069- ABflo647 | 200 T | 5 μL |
| CD8a | A23905 | ABflo® 594 | 588/604 | Rabbit IgG | ARC60677- ABflo594 | 200 T | 5 μL |
| CD161c/NK1.1 | A24107 | PE | 565/574 | Rabbit IgG | ARC57668-PE | 200 T | 5 μL |
Note:
This reagent has been pre-diluted for use at the recommended Volume per Test. We typically use 1×10^6 cells in a 100 µl experimental sample (a test).
CD3e: The protein encoded by this gene is the CD3-epsilon polypeptide, which together with CD3-gamma, -delta and -zeta, and the T-cell receptor alpha/beta and gamma/delta heterodimers, forms the T-cell receptor-CD3 complex. This complex plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways.
CD4: Is a 55 kDa transmembrane glycoprotein expressed on helper T lymphocytes. CD4⁺T cells are crucial in achieving a regulated effective immune response to pathogens.
CD8a: Is a member of T cytotoxic pathway-related genes and encodes the CD8 antigen that is a cell surface glycoprotein found on most cytotoxic T cells. CD8⁺ T-cells are important effector cells in specific cell-mediated antiviral immunity.
CD161c/NK1.1: Is also known as killer cell lectin-like receptor subfamily B member 1C (Klrb1c), is a marker of mouse NK cells, but only in certain strains, including C57BL/6, FVB/N, and NZB.
首先，一般抗体不推荐客户回收利用，抗体使用之后缓冲体系已经发生改变，不同客户在回收抗体的保存条件上也会有差异，所以抗体回收使用效果无法保证。另外，ABclonal公司也做过一批抗体回收验证测试，测试结果显示不同抗体可回收次数不同，一般效价越高的抗体，可重复使用的次数越多，客户可根据实验情况来确定。
注：我们将孵育完毕后剩余的抗体回收到离心管中置于4℃保存，效价高的抗体可至少保存1周，至少重复利用3次。
武汉爱博泰克生物（ABclonal）科技有限公司是国产品牌，她成立于2011年，公司依托ABclonal美国波士顿抗体与蛋白研发中心、中国光谷生物城(武汉)抗体生产基地以及上海张江分子酶研发中心，凝聚了十余位来自哈佛大学、麻省理工、复旦大学、上海交大、中科院生化细胞所和武汉大学的全球知名分子和免疫学方面博士，组成我们的科学家团队，通过聚焦抗体与酶核心技术，致力于打破国际技术的垄断，将公司打造成为科研工具和诊断原料的国内领导品牌，乃至弯道超越国际巨头。 我们拥有包括兔多克隆抗体、小鼠单克隆抗体、兔单克隆抗体的生产研发平台，同时也有包括WB,IHC,IF,IP,CHIP在内的检测平台，我们对每一支自产的抗体进行了严格的检测。当然，我们部分直销地区也可以帮客户代购进口品牌的产品。同时也有抗体定制服务。ABclonal抗体优势：1，严自检，保质量；2产品多，指标全；3，价格低，货期短。注：ABclonal抗体价格体系详情见附件
ABclonal抗体成分在平时工作当中，常会有客户咨询我们的抗体用的什么buffer进行保存，一般来说，我们的buffer的成分是：PBS含0.03%的proclin300、0.05%牛血清白蛋白、50%甘油；也有一些是PBS含0.03%的proclin300，50%甘油。防腐剂 Proclin 300活性成分主要是2-甲基-4-异噻唑啉-3-酮（MCI）和5-氯-2-甲基-4-异噻唑啉-3-酮（CMCI）。ProClin生物灭活剂能够迅速穿透细胞膜，抑制对细胞呼吸至关重要的特定酶，因此一接触微生物有机体就会立即抑制细胞活性。ProClin的多个特定毒性位点可以防止微生物产生高水平的耐药性。
