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NF-kB p65/RelA Rabbit pAb (A2547)

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Western blot analysis of various lysates using NF-kB p65/RelA Rabbit pAb (A2547) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (RM00020).Exposure time: 180s.

Western blot analysis of various lysates, using NF-kB p65/RelA Rabbit pAb (A2547) at 1:2000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (RM00020).Exposure time: 20s.

Western blot analysis of lysates from wild type(WT) and NF-kB p65/RelA knockout (KO) HeLa(KO) cells, using NF-kB p65/RelA Rabbit pAb (A2547) at 1:2000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (RM00020).Exposure time: 20s.

Immunohistochemistry analysis of paraffin-embedded human colon using [KO Validated] NF-kB p65/RelA Rabbit pAb (A2547) at dilution of 1:200 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

Immunohistochemistry analysis of paraffin-embedded mouse kidney using [KO Validated] NF-kB p65/RelA Rabbit pAb (A2547) at dilution of 1:200 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

Immunohistochemistry analysis of paraffin-embedded rat testis using [KO Validated] NF-kB p65/RelA Rabbit pAb (A2547) at dilution of 1:200 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

Immunohistochemistry analysis of paraffin-embedded human colon using NF-kB p65/RelA Rabbit pAb (A2547) at dilution of 1:300 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

Immunohistochemistry analysis of paraffin-embedded mouse colon using NF-kB p65/RelA Rabbit pAb (A2547) at dilution of 1:300 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

Immunohistochemistry analysis of paraffin-embedded rat heart using NF-kB p65/RelA Rabbit pAb (A2547) at dilution of 1:300 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

Immunofluorescence analysis of HepG2 cells using [KO Validated] NF-kB p65/RelA Rabbit pAb (A2547) at dilution of 1:200 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

Immunofluorescence analysis of NIH/3T3 cells using [KO Validated] NF-kB p65/RelA Rabbit pAb (A2547) at dilution of 1:200 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

Immunofluorescence analysis of HepG2 cells using NF-kB p65/RelA Rabbit pAb (A2547) at dilution of 1:200 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

Immunofluorescence analysis of NIH/3T3 cells using NF-kB p65/RelA Rabbit pAb (A2547) at dilution of 1:200 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

All(13)|
货号: A2547
促销价：   ￥1150
货    期：现货产品
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文献引用 (142)

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Publishing research using A2547? Please let us know so that we can cite the reference in this datasheet.

客户数据及评论 (6)

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CustomerJul 152017
评分
Experiment Type
Western blot(WB)
Sample
RAW264.7
Description
Western blot analysis of extracts of mouse atherosclerosis arterial tissue, using RELA antibody (A2547) at 1:500 dilution.The band is specific and clear.
CustomerJul 102017
评分
Experiment Type
Western blot(WB)
Sample
Mouse atherosclerosis arterial tissue
Description
Western blot analysis of extracts of Mouse atherosclerosis arterial tissue, using RELA antibody (A2547) at 1:500 dilution.The band is specific and clear.
CustomerJun 252015
评分
Experiment Type
Western blot(WB)
Sample
HCC827
Description
Western blot analysis of extracts of HCC827 cells, using RELA Polyclonal Antibody.
CustomerJun 252015
评分
Experiment Type
Western blot(WB)
Sample
Raw264.7
Description
Western blot analysis of extracts of Raw264.7 cells, using RELA Polyclonal Antibody.
CustomerJun 242015
评分
Experiment Type
Western blot(WB)
Sample
U251
Description
Western blot analysis of extracts of U251 cells, using RELA Polyclonal Antibody.
CustomerJun 212015
评分
Experiment Type
Immunofluorescence(IF)
Sample
Rat H9c2 cells
Description
Immunofluorescence analysis of Rat H9c2 cells using P65 Antibody 1:100 .

详细信息

推荐稀释比
  • WB1:500 - 1:2000
  • IHC-P1:100 - 1:500
  • IF/ICC1:50 - 1:200
浓度查询

请输入产品标签上的lot号，例如4000000001

反应物种
Human, Mouse, Rat
同种型
IgG
理论分子量
60kDa
实际分子量
65kDa
产品形式
Liquid
偶联物
Unconjugated
存储缓冲液
Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
阳性样本
HeLa,293T,MCF7,Mouse lung,Mouse kidney,Rat lung,Rat kidney
细胞定位
Cytoplasm,Nucleus
纯化方式
Affinity purification

抗原信息

免疫原
Recombinant fusion protein containing a sequence corresponding to amino acids 450-550 of human NF-kB p65/RelA (NP_068810.3).
序列
LGALLGNSTDPAVFTDLASVDNSEFQQLLNQGIPVAPHTTEPMLMEYPEAITRLVTGAQRPPDPAPAPLGAPGLPNGLLSGDEDFSSIADMDFSALLSQIS
进行序列比对

用户验证的应用

应用及物种
  • WB (RAW264.7 , Mouse atherosclerosis arterial tissue , HCC827 , U251Mus musculus , Mus musculus , Homo sapiens , Homo sapiens , Mus musculus , Sus scrofa , Rattus norvegicus , King Charles Spaniel , Schisandra chinensis , Gallus gallus , Macaca mulatta , Cryptosporidium parvum , Alpinia katsumadai Hayata , Bos taurus)
  • IF (Rat H9c2 cellsHomo sapiens , Mus musculus , Rattus norvegicus , Homo sapiens , Homo sapiens , Mus musculus)
  • IHC (Mus musculus , Rattus norvegicus , Homo sapiens)
  • ChIP (Homo sapiens)
  • IB (Homo sapiens)
  • PE (Mus musculus)

背景信息

NF-kappa-B is a ubiquitous transcription factor involved in several biological processes. It is held in the cytoplasm in an inactive state by specific inhibitors. Upon degradation of the inhibitor, NF-kappa-B moves to the nucleus and activates transcription of specific genes. NF-kappa-B is composed of NFKB1 or NFKB2 bound to either REL, RELA, or RELB. The most abundant form of NF-kappa-B is NFKB1 complexed with the product of this gene, RELA. Four transcript variants encoding different isoforms have been found for this gene.

RRID
AB_2764436
基因ID
5970
Swiss Prot
Q04206
别名
p65; CMCU; NFKB3; AIF3BL3; NF-kB p65/RelA
研究领域

关联靶点

以RELA为关键词搜索文献记录，得到上述基因与RELA的研究紧密相关，排序顺序根据研究相关程度由高到低决定。（所显示数字代表两者共同出现的已发表文献数量）
◀1/▶

关联领域

以RELA为关键词搜索文献记录，得到上述相关热词信息，可反映出RELA的研究趋势与热点。（关联领域与基因名称的距离代表其研究热度）

* 该数据来源于赛特新思(citexs.com)
来自于发表文献，仅供参考所用，ABclonal不承担任何责任。

暂无以RELA为关键词搜索的文献记录。

该服务由赛特新思提供，上述关联分析来自已发表文献，仅供参考所用，ABclonal不承担任何责任。

FAQ

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ABclonal公司的抗体，可以回收利用几次？

首先，一般抗体不推荐客户回收利用，抗体使用之后缓冲体系已经发生改变，不同客户在回收抗体的保存条件上也会有差异，所以抗体回收使用效果无法保证。另外，ABclonal公司也做过一批抗体回收验证测试，测试结果显示不同抗体可回收次数不同，一般效价越高的抗体，可重复使用的次数越多，客户可根据实验情况来确定。
注：我们将孵育完毕后剩余的抗体回收到离心管中置于4℃保存，效价高的抗体可至少保存1周，至少重复利用3次。

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ABclonal作为国产抗体品牌的优势？

武汉爱博泰克生物（ABclonal）科技有限公司是国产品牌，她成立于2011年，公司依托ABclonal美国波士顿抗体与蛋白研发中心、中国光谷生物城(武汉)抗体生产基地以及上海张江分子酶研发中心，凝聚了十余位来自哈佛大学、麻省理工、复旦大学、上海交大、中科院生化细胞所和武汉大学的全球知名分子和免疫学方面博士，组成我们的科学家团队，通过聚焦抗体与酶核心技术，致力于打破国际技术的垄断，将公司打造成为科研工具和诊断原料的国内领导品牌，乃至弯道超越国际巨头。 我们拥有包括兔多克隆抗体、小鼠单克隆抗体、兔单克隆抗体的生产研发平台，同时也有包括WB,IHC,IF,IP,CHIP在内的检测平台，我们对每一支自产的抗体进行了严格的检测。当然，我们部分直销地区也可以帮客户代购进口品牌的产品。同时也有抗体定制服务。ABclonal抗体优势：1，严自检，保质量；2产品多，指标全；3，价格低，货期短。注：ABclonal抗体价格体系详情见附件

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ABclonal抗体成分？

ABclonal抗体成分在平时工作当中，常会有客户咨询我们的抗体用的什么buffer进行保存，一般来说，我们的buffer的成分是：PBS含0.03%的proclin300、0.05%牛血清白蛋白、50%甘油；也有一些是PBS含0.03%的proclin300，50%甘油。防腐剂 Proclin 300活性成分主要是2-甲基-4-异噻唑啉-3-酮（MCI）和5-氯-2-甲基-4-异噻唑啉-3-酮（CMCI）。ProClin生物灭活剂能够迅速穿透细胞膜，抑制对细胞呼吸至关重要的特定酶，因此一接触微生物有机体就会立即抑制细胞活性。ProClin的多个特定毒性位点可以防止微生物产生高水平的耐药性。

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实验方案

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